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Intron retention in the 5'UTR of the novel ZIF2 transporter enhances translation to promote zinc tolerance in arabidopsis

2014-05-15Journal article Open access
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dc.contributor.authorRemy, Estelle
dc.contributor.authorCabrito, Tânia R
dc.contributor.authorBatista, Rita A
dc.contributor.authorHussein, Mohamed A M
dc.contributor.authorTeixeira, Miguel C
dc.contributor.authorAthanasiadis, Alekos
dc.contributor.authorSá-Correia, Isabel
dc.contributor.authorDuque, Paula
dc.date.accessioned2015-10-01T15:42:44Z
dc.date.available2015-10-01T15:42:44Z
dc.date.issued2014-05-15
dc.description.abstractRoot vacuolar sequestration is one of the best-conserved plant strategies to cope with heavy metal toxicity. Here we report that zinc (Zn) tolerance in Arabidopsis requires the action of a novel Major Facilitator Superfamily (MFS) transporter. We show that ZIF2 (Zinc-Induced Facilitator 2) localises primarily at the tonoplast of root cortical cells and is a functional transporter able to mediate Zn efflux when heterologously expressed in yeast. By affecting plant tissue partitioning of the metal ion, loss of ZIF2 function exacerbates plant sensitivity to excess Zn, while its overexpression enhances Zn tolerance. The ZIF2 gene is Zn-induced and an intron retention event in its 5'UTR generates two splice variants (ZIF2.1 and ZIF2.2) encoding the same protein. Importantly, high Zn favours production of the longer ZIF2.2 transcript, which compared to ZIF2.1 confers greater Zn tolerance to transgenic plants by promoting higher root Zn immobilization. We show that the retained intron in the ZIF2 5'UTR enhances translation in a Zn-responsive manner, markedly promoting ZIF2 protein expression under excess Zn. Moreover, Zn regulation of translation driven by the ZIF2.2 5'UTR depends largely on a predicted stable stem loop immediately upstream of the start codon that is lost in the ZIF2.1 5'UTR. Collectively, our findings indicate that alternative splicing controls the levels of a Zn-responsive mRNA variant of the ZIF2 transporter to enhance plant tolerance to the metal ion.pt_PT
dc.description.sponsorshipFCT PostDoctoral Fellowships: SFRH/BPD/44640/2008, SFRH/BPD/81221/2011.pt_PT
dc.identifier10.1371/journal.pgen.1004375
dc.identifier.citationRemy E, Cabrito TR, Batista RA, Hussein MAM, Teixeira MC, Athanasiadis A, et al. (2014) Intron Retention in the 5′UTR of the Novel ZIF2 Transporter Enhances Translation to Promote Zinc Tolerance in Arabidopsis. PLoS Genet 10(5): e1004375. doi:10.1371/journal.pgen.1004375pt_PT
dc.identifier.doi10.1371/journal.pgen.1004375
dc.identifier.doi10.1371/journal.pgen.1004375
dc.identifier.urihttp://hdl.handle.net/10400.7/336
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPLOSpt_PT
dc.relation.publisherversionhttp://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1004375pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectZincpt_PT
dc.subjectIntronspt_PT
dc.subjectArabidopsis thalianapt_PT
dc.titleIntron retention in the 5'UTR of the novel ZIF2 transporter enhances translation to promote zinc tolerance in arabidopsispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-AAM%2F102967%2F2008/PT
oaire.citation.endPage19pt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titlePLOS Geneticspt_PT
oaire.citation.volume10pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication87d8831f-5f09-40d2-bff7-79c374b5f000
relation.isProjectOfPublication.latestForDiscovery87d8831f-5f09-40d2-bff7-79c374b5f000

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