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A DNA Metabarcoding Study of a Primate Dietary Diversity and Plasticity across Its Entire Fragmented Range

2013-03-19Journal article Open access
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dc.contributor.authorQuéméré, Erwan
dc.contributor.authorHibert, Fabrice
dc.contributor.authorMiquel, Christian
dc.contributor.authorLhuillier, Emeline
dc.contributor.authorRasolondraibe, Emmanuel
dc.contributor.authorChampeau, Julie
dc.contributor.authorRabarivola, Clément
dc.contributor.authorNusbaumer, Louis
dc.contributor.authorChatelain, Cyrille
dc.contributor.authorGautier, Laurent
dc.contributor.authorRanirison, Patrick
dc.contributor.authorCrouau-Roy, Brigitte
dc.contributor.authorTaberlet, Pierre
dc.contributor.authorChikhi, Lounès
dc.date.accessioned2015-11-06T17:20:11Z
dc.date.available2015-11-06T17:20:11Z
dc.date.issued2013-03-19
dc.description.abstractIn tropical regions, most primary ecosystems have been replaced by mosaic landscapes in which species must cope with a large shift in the distribution of their habitat and associated food resources. Primates are particularly vulnerable to habitat modifications. Most species persist in small fragments surrounded by complex human-mediated matrices whose structure and connectivity may strongly influence their dispersal and feeding behavior. Behavioral plasticity appears to be a crucial parameter governing the ability of organisms to exploit the resources offered by new matrix habitats and thus to persist in fragmented habitats. In this study, we were interested in the dietary plasticity of the golden-crowned sifaka (Propithecus tattersalli), an endangered species of lemur, found only in the Daraina region in north-eastern Madagascar. We used a DNA-based approach combining the barcoding concept and Illumina next-generation sequencing to (i) describe the species diet across its entire range and (ii) evaluate the influence of landscape heterogeneity on diet diversity and composition. Faeces from 96 individuals were sampled across the entire species range and their contents were analyzed using the trnL metabarcoding approach. In parallel, we built a large DNA reference database based on a checklist of the plant species of the Daraina region. Our results suggest that golden-crowned sifakas exhibit remarkable dietary diversity with at least 130 plant species belonging to 80 genera and 49 different families. We highlighted an influence of both habitat type and openness on diet composition suggesting a high flexibility of foraging strategies. Moreover, we observed the presence of numerous cultivated and naturalized plants in the faeces of groups living in forest edge areas. Overall, our findings support our initial expectation that P. tattersalli is able to cope with the current level of alteration of the landscape and confirm our previous results on the distribution and the dispersal ability of this species.pt_PT
dc.description.sponsorshipInstitut Francais de la Biodiversité, Programme Biodiversité de l'Océan Indien (reference no.CD-AOO1-07-003), Laboratoire d’Excellence (LABEX) entitled TULIP (ANR-10-LABX-41), Conservatoire et Jardin botaniques de la Ville de Genève, Conservation International (CBC fund), Fondation Jean-Marcel Aubert, Vontobel Stiftung.pt_PT
dc.identifier10.1371/journal.pone.0058971
dc.identifier.citationQue ́ me ́ re ́ E, Hibert F, Miquel C, Lhuillier E, Rasolondraibe E, et al. (2013) A DNA Metabarcoding Study of a Primate Dietary Diversity and Plasticity across Its Entire Fragmented Range. PLoS ONE 8(3): e58971. doi:10.1371/journal.pone.0058971pt_PT
dc.identifier.doi10.1371/journal.pone.0058971
dc.identifier.urihttp://hdl.handle.net/10400.7/475
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPLOSpt_PT
dc.relation.publisherversionhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0058971pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSequence databasespt_PT
dc.subjectForestspt_PT
dc.subjectHabitatspt_PT
dc.subjectDietpt_PT
dc.subjectPrimatespt_PT
dc.subjectDNA sequence analysispt_PT
dc.subjectLeavespt_PT
dc.subjectPolymerase chain reactionpt_PT
dc.titleA DNA Metabarcoding Study of a Primate Dietary Diversity and Plasticity across Its Entire Fragmented Rangept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-BEC%2F100176%2F2008/PT
oaire.citation.endPage11pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titlePLOS Onept_PT
oaire.citation.volume8pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicatione097521b-ad8c-4626-a074-aeb1cb70c4bd
relation.isProjectOfPublication.latestForDiscoverye097521b-ad8c-4626-a074-aeb1cb70c4bd

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