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Cell- and Tissue-Specific Transcriptome Analyses of Medicago truncatula Root Nodules

2013-05-29Journal article Open access
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dc.contributor.authorLimpens, Erik
dc.contributor.authorMoling, Sjef
dc.contributor.authorHooiveld, Guido
dc.contributor.authorPereira, Patrícia A.
dc.contributor.authorBisseling, Ton
dc.contributor.authorBecker, Jörg D.
dc.contributor.authorKüster, Helge
dc.date.accessioned2015-11-02T15:43:32Z
dc.date.available2015-11-02T15:43:32Z
dc.date.issued2013-05-29
dc.description.abstractLegumes have the unique ability to host nitrogen-fixing Rhizobium bacteria as symbiosomes inside root nodule cells. To get insight into this key process, which forms the heart of the endosymbiosis, we isolated specific cells/tissues at different stages of symbiosome formation from nodules of the model legume Medicago truncatula using laser-capture microdissection. Next, we determined their associated expression profiles using Affymetrix Medicago GeneChips. Cells were collected from the nodule infection zone divided into a distal (where symbiosome formation and division occur) and proximal region (where symbiosomes are mainly differentiating), as well as infected cells from the fixation zone containing mature nitrogen fixing symbiosomes. As non-infected cells/tissue we included nodule meristem cells and uninfected cells from the fixation zone. Here, we present a comprehensive gene expression map of an indeterminate Medicago nodule and selected genes that show specific enriched expression in the different cells or tissues. Validation of the obtained expression profiles, by comparison to published gene expression profiles and experimental verification, indicates that the data can be used as digital "in situ". This digital "in situ" offers a genome-wide insight into genes specifically associated with subsequent stages of symbiosome and nodule cell development, and can serve to guide future functional studies.pt_PT
dc.description.sponsorshipDutch Organization for Scientific Research grant: (184319448).pt_PT
dc.identifier.citationLimpens E, Moling S, Hooiveld G, Pereira PA, Bisseling T, Becker JD, et al. (2013) Cell- and Tissue-Specific Transcriptome Analyses of Medicago truncatula Root Nodules. PLoS ONE 8(5): e64377. doi:10.1371/journal.pone.0064377pt_PT
dc.identifier.doi10.1371/journal.pone.0064377
dc.identifier.urihttp://hdl.handle.net/10400.7/457
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPLOSpt_PT
dc.relationExpression profiling studies to specify the symbiotic genetic programme of the model legume Medicago truncatula
dc.relation.publisherversionhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0064377pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCellspt_PT
dc.subjectGene Expression Profilingpt_PT
dc.subjectMedicago truncatulapt_PT
dc.subjectRoot Nodules, Plantpt_PT
dc.titleCell- and Tissue-Specific Transcriptome Analyses of Medicago truncatula Root Nodulespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleExpression profiling studies to specify the symbiotic genetic programme of the model legume Medicago truncatula
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-GPL%2F70592%2F2006/PT
oaire.citation.endPage15pt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titlePLOS Onept_PT
oaire.citation.volume8pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationb4c8cc78-8600-4b33-8318-140b9a4fa827
relation.isProjectOfPublication.latestForDiscoveryb4c8cc78-8600-4b33-8318-140b9a4fa827

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