Repository logo
 
Publication

 -Tubulin-containing abnormal centrioles are induced by insufficient Plk4 in human HCT116 colorectal cancer cells

2009-06-03Journal article Open access
Simple item page
dc.contributor.authorKuriyama, R.
dc.contributor.authorBettencourt-Dias, M.
dc.contributor.authorHoffmann, I.
dc.contributor.authorArnold, M.
dc.contributor.authorSandvig, L.
dc.date.accessioned2017-05-08T16:09:06Z
dc.date.available2017-05-08T16:09:06Z
dc.date.issued2009-06-03
dc.descriptionThe article hasn't a creative commons license associated nor informations about funders/sponsors. There is no public supplementary material available.pt_PT
dc.description.abstractCancer cells frequently induce aberrant centrosomes, which have been implicated in cancer initiation and progression. Human colorectal cancer cells, HCT116, contain aberrant centrioles composed of disorganized cylindrical microtubules and displaced appendages. These cells also express unique centrosome-related structures associated with a subset of centrosomal components, including gamma-tubulin, centrin and PCM1. During hydroxyurea treatment, these abnormal structures become more abundant and undergo a change in shape from small dots to elongated fibers. Although gamma-tubulin seems to exist as a ring complex, the abnormal structures do not support microtubule nucleation. Several lines of evidence suggest that the fibers correspond to a disorganized form of centriolar microtubules. Plk4, a mammalian homolog of ZYG-1 essential for initiation of centriole biogenesis, is not associated with the gamma-tubulin-specific abnormal centrosomes. The amount of Plk4 at each centrosome was less in cells with abnormal centrosomes than cells without gamma-tubulin-specific abnormal centrosomes. In addition, the formation of abnormal structures was abolished by expression of exogenous Plk4, but not SAS6 and Cep135/Bld10p, which are downstream regulators required for the organization of nine-triplet microtubules. These results suggest that HCT116 cells fail to organize the ninefold symmetry of centrioles due to insufficient Plk4.pt_PT
dc.description.sponsorshipThere are no funders and sponsors indicated explicitly in the document.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGamma -Tubulin-containing abnormal centrioles are induced by insufficient Plk4 in human HCT116 colorectal cancer cells Ryoko Kuriyama, Monica Bettencourt-Dias, Ingrid Hoffmann, Marc Arnold, Lisa Sandvig J Cell Sci 2009 122: 2014-2023; doi: 10.1242/jcs.036715pt_PT
dc.identifier.doi10.1242/jcs.036715pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/752
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherCompany of Biologistspt_PT
dc.relationThere are no funders and sponsors indicated explicitly in the document.pt_PT
dc.relation.publisherversionhttp://jcs.biologists.org/content/122/12/2014pt_PT
dc.subjectCentriolespt_PT
dc.subjectColorectal Neoplasmspt_PT
dc.subjectHumanspt_PT
dc.subjectHydroxyureapt_PT
dc.subjectMicrotubulespt_PT
dc.subjectNocodazolept_PT
dc.subjectProtein-Serine-Threonine Kinasespt_PT
dc.subjectSpindle Apparatuspt_PT
dc.subjectTubulinpt_PT
dc.subjectTubulin Modulatorspt_PT
dc.subjectUp-Regulationpt_PT
dc.subjectHCT116 Cellspt_PT
dc.title -Tubulin-containing abnormal centrioles are induced by insufficient Plk4 in human HCT116 colorectal cancer cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage2023pt_PT
oaire.citation.issue12pt_PT
oaire.citation.startPage2014pt_PT
oaire.citation.titleJournal of Cell Sciencept_PT
oaire.citation.volume122pt_PT
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
J Cell Sci 2009_ 122_ 2014-2023.pdf
Size:
2.38 MB
Format:
Adobe Portable Document Format
Description:
main article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCR - Artigos