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Irf4 is a positional and functional candidate gene for the control of serum IgM levels in the mouse

2009Journal article Open access
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dc.contributor.authorCôrte-Real, J
dc.contributor.authorRodo, Joana
dc.contributor.authorAlmeida, P
dc.contributor.authorCoutinho, António
dc.contributor.authorDemengeot, Jocelyne
dc.contributor.authorPenha-Gonçalves, Carlos
dc.date.accessioned2010-05-14T10:16:47Z
dc.date.available2010-05-14T10:16:47Z
dc.date.issued2009
dc.description.abstractNatural IgM are involved in numerous immunological functions but the genetic factors that control the homeostasis of its secretion and upholding remain unknown. Prompted by the finding that C57BL/6 mice had significantly lower serum levels of IgM when compared with BALB/c mice, we performed a genome-wide screen and found that the level of serum IgM was controlled by a QTL on chromosome 13 reaching the highest level of association at marker D13Mit266 (LOD score¼3.54). This locus was named IgMSC1 and covered a region encompassing the interferon-regulatory factor 4 gene (Irf4). The number of splenic mature B cells in C57BL/6 did not differ from BALB/c mice but we found that low serum levels of IgM in C57BL/6 mice correlated with lower frequency of IgM-secreting cells in the spleen and in the peritoneal cavity. These results suggested that C57BL/6 mice have lower efficiency in late B-cell maturation, a process that is highly impaired in Irf4 knockout mice. In fact, we also found reduced Irf4 gene expression in B cells of C57BL/6 mice. Thus, we propose Irf4 as a candidate for the IgMSC1 locus, which controls IgM homeostatic levels at the level of B-cell terminal differentiation.pt
dc.identifier.citationCôrte-Real, J., Rodo, J., Almeida, P., Garcia, J., Coutinho, A., Demengeot, J., Penha Gonçalves, C. (2009). Irf4 is a positional and functional candidate gene for the control of serum IgM levels in the mouse. Genes Immun 10(1) :1-7pt
dc.identifier.issn1466-4879
dc.identifier.urihttp://hdl.handle.net/10400.7/112
dc.language.isoengpt
dc.peerreviewedYes
dc.publisherNaturept
dc.relation.publisherversionhttp://dx.doi.org/10.1038/gene.2008.73
dc.subjectSerum IgMpt
dc.subjecthomeostasispt
dc.subjectIRF4pt
dc.titleIrf4 is a positional and functional candidate gene for the control of serum IgM levels in the mousept
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage99pt
oaire.citation.startPage93pt
oaire.citation.titleGenes and Immunitypt
rcaap.rightsopenAccesspt
rcaap.typearticlept

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