Comparative analysis of transcriptomic responses to sub-lethal levels of six environmentally relevant pesticides in Saccharomyces cerevisiae

Gil, Fátima N.; Gonçalves, Alina C.; Becker, Jörg D.; Viegas, Cristina A.

Publication

Comparative analysis of transcriptomic responses to sub-lethal levels of six environmentally relevant pesticides in Saccharomyces cerevisiae

2018-04Journal article

dc.contributor.author	Gil, Fátima N.
dc.contributor.author	Gonçalves, Alina C.
dc.contributor.author	Becker, Jörg D.
dc.contributor.author	Viegas, Cristina A.
dc.date.accessioned	2018-04-09T17:20:52Z
dc.date.available	2018-04-09T17:20:52Z
dc.date.issued	2018-04
dc.description	This deposit is composed by a publication in which the IGC's authors have had the role of collaboration (it's a collaboration publication). This type of deposit in ARCA is in restrictedAccess (it can't be in open access to the public), and can only be accessed by two ways: either by requesting a legal copy from the author (the email contact present in this deposit) or by visiting the following link: https://link.springer.com/article/10.1007%2Fs10646-018-1929-1	pt_PT
dc.description.abstract	Accidental spills and misuse of pesticides may lead to current and/or legacy environmental contamination and may pose concerns regarding possible risks towards non-target microbes and higher eukaryotes in ecosystems. The present study was aimed at comparing transcriptomic responses to effects of sub-lethal levels of six environmentally relevant pesticide active substances in the Saccharomyces cerevisiae eukaryotic model. The insecticide carbofuran, the fungicide pyrimethanil and the herbicides alachlor, S-metolachlor, diuron and methyl(4-chloro-2-methylphenoxy)acetate were studied. Some are currently used agricultural pesticides, while others are under restricted utilization or banned in Europe and/or North America albeit being used in other geographical locations. In the present work transcriptional profiles representing genome-wide responses in a standardized yeast population upon 2 h of exposure to concentrations of each compound exerting equivalent toxic effects, i.e., inhibition of growth by 20% relative to the untreated control cells, were examined. Hierarchical clustering and Venn analyses of the datasets of differentially expressed genes pointed out transcriptional patterns distinguishable between the six active substances. Functional enrichment analyses allowed predicting mechanisms of pesticide toxicity and response to pesticide stress in the yeast model. In general, variations in transcript numbers of selected genes assessed by Real-Time quantitative reverse transcription polymerase chain reaction confirmed microarray data and correlated well with growth inhibitory effects. A possible biological relevance of mechanistic predictions arising from these comparative transcriptomic analyses is discussed in the context of better understanding potential modes of action and adverse side-effects of pesticides.	pt_PT
dc.description.sponsorship	Fundação para a Ciência e Tecnologia grants:(UID/BIO/04565/2013, PTDC/AMB/64230/2006, SFRH/BD/60933/2009); POR Lisboa 2020 grant: (LISBOA-01-0145-FEDER-007317); FEDER; COMPETE.	pt_PT
dc.description.version	info:eu-repo/semantics/acceptedVersion	pt_PT
dc.identifier.citation	Gil, F.N., Gonçalves, A.C., Becker, J.D. et al. Ecotoxicology (2018). https://doi.org/10.1007/s10646-018-1929-1	pt_PT
dc.identifier.doi	10.1007/s10646-018-1929-1	pt_PT
dc.identifier.uri	http://hdl.handle.net/10400.7/859
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	Springer Verlag	pt_PT
dc.relation	LISBOA-01-0145-FEDER-007317	pt_PT
dc.relation	IDENTIFICATION OF MOLECULAR BIOMARKERS RELEVANT FOR ENVIRONMENTAL BIOMONITORING OF PESTICIDE TOXICITY USING SIMPLE EUKARYOTIC MODELS
dc.relation.publisherversion	https://link.springer.com/article/10.1007%2Fs10646-018-1929-1	pt_PT
dc.subject	Pesticide	pt_PT
dc.subject	Sub-lethal	pt_PT
dc.subject	Toxicogenomics	pt_PT
dc.subject	Toxicity mechanisms	pt_PT
dc.subject	Molecular response	pt_PT
dc.subject	Saccharomyces cerevisiae	pt_PT
dc.title	Comparative analysis of transcriptomic responses to sub-lethal levels of six environmentally relevant pesticides in Saccharomyces cerevisiae	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.awardTitle	IDENTIFICATION OF MOLECULAR BIOMARKERS RELEVANT FOR ENVIRONMENTAL BIOMONITORING OF PESTICIDE TOXICITY USING SIMPLE EUKARYOTIC MODELS
oaire.awardURI	info:eu-repo/grantAgreement/FCT/5876/UID%2FBIO%2F04565%2F2013/PT
oaire.awardURI	info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAMB%2F64230%2F2006/PT
oaire.awardURI	info:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F60933%2F2009/PT
oaire.citation.endPage	19	pt_PT
oaire.citation.startPage	1	pt_PT
oaire.citation.title	Ecotoxicology	pt_PT
oaire.fundingStream	5876
oaire.fundingStream	3599-PPCDT
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.name	Fundação para a Ciência e a Tecnologia
project.funder.name	Fundação para a Ciência e a Tecnologia
project.funder.name	Fundação para a Ciência e a Tecnologia
rcaap.rights	restrictedAccess	pt_PT
rcaap.type	article	pt_PT
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