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Rootletin organizes the ciliary rootlet to achieve neuron sensory function in Drosophila

2015-10-26Journal article Open access
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dc.contributor.authorChen, Jieyan V
dc.contributor.authorKao, Ling-Rong
dc.contributor.authorJana, Swadhin C
dc.contributor.authorSivan-Loukianova, Elena
dc.contributor.authorMendonça, Susana
dc.contributor.authorCabrera, Oscar A
dc.contributor.authorSingh, Priyanka
dc.contributor.authorCabernard, Clemens
dc.contributor.authorEberl, Daniel F
dc.contributor.authorBettencourt-Dias, Monica
dc.contributor.authorMegraw, Timothy L
dc.date.accessioned2015-11-17T12:30:05Z
dc.date.available2015-11-17T12:30:05Z
dc.date.issued2015-10-26
dc.description.abstractCilia are essential for cell signaling and sensory perception. In many cell types, a cytoskeletal structure called the ciliary rootlet links the cilium to the cell body. Previous studies indicated that rootlets support the long-term stability of some cilia. Here we report that Drosophila melanogaster Rootletin (Root), the sole orthologue of the mammalian paralogs Rootletin and C-Nap1, assembles into rootlets of diverse lengths among sensory neuron subtypes. Root mutant neurons lack rootlets and have dramatically impaired sensory function, resulting in behavior defects associated with mechanosensation and chemosensation. Root is required for cohesion of basal bodies, but the cilium structure appears normal in Root mutant neurons. We show, however, that normal rootlet assembly requires centrioles. The N terminus of Root contains a conserved domain and is essential for Root function in vivo. Ectopically expressed Root resides at the base of mother centrioles in spermatocytes and localizes asymmetrically to mother centrosomes in neuroblasts, both requiring Bld10, a basal body protein with varied functions.pt_PT
dc.description.sponsorshipNational Institutes of Health grant: (GM068758).pt_PT
dc.identifier.citationRootletin organizes the ciliary rootlet to achieve neuron sensory function in Drosophila Jieyan V. Chen, Ling-Rong Kao, Swadhin C. Jana, Elena Sivan-Loukianova, Susana Mendonça, Oscar A. Cabrera, Priyanka Singh, Clemens Cabernard, Daniel F. Eberl, Monica Bettencourt-Dias, and Timothy L. Megraw J Cell Biol 2015 211:435-453. Published October 19, 2015, doi:10.1083/jcb.201502032pt_PT
dc.identifier.doi10.1083/jcb.201502032pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/496
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherThe Rockefeller University Presspt_PT
dc.relation.publisherversionhttp://jcb.rupress.org/content/211/2/435.longpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/pt_PT
dc.subjectDrosophila melanogasterpt_PT
dc.subjectCiliary Rootletpt_PT
dc.subjectNeuron Sensorypt_PT
dc.titleRootletin organizes the ciliary rootlet to achieve neuron sensory function in Drosophilapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage453pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage435pt_PT
oaire.citation.titleThe Journal of Cell Biologypt_PT
oaire.citation.volume211pt_PT
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT

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