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HydroxyprolineO-arabinosyltransferase mutants oppositely alter tip growth inArabidopsis thalianaandPhyscomitrella patens

2016-01Journal article Open access
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dc.contributor.authorMacAlister, Cora A.
dc.contributor.authorOrtiz-Ramírez, Carlos
dc.contributor.authorBecker, Jörg D.
dc.contributor.authorFeijó, José A.
dc.contributor.authorLippman, Zachary B.
dc.date.accessioned2016-11-16T15:08:41Z
dc.date.available2017-01-01T01:30:08Z
dc.date.issued2016-01
dc.description.abstractHydroxyproline O-arabinosyltransferases (HPATs) are members of a small, deeply conserved family of plant-specific glycosyltransferases that add arabinose sugars to diverse proteins including cell wall-associated extensins and small signaling peptides. Recent genetic studies in flowering plants suggest that different HPAT homologs have been co-opted to function in diverse species-specific developmental contexts. However, nothing is known about the roles of HPATs in basal plants. We show that complete loss of HPAT function in Arabidopsis thaliana and the moss Physcomitrella patens results in a shared defect in gametophytic tip cell growth. Arabidopsis hpat1/2/3 triple knockout mutants suffer from a strong male sterility defect as a consequence of pollen tubes that fail to fully elongate following pollination. Knocking out the two HPAT genes of Physcomitrella results in larger multicellular filamentous networks due to increased elongation of protonemal tip cells. Physcomitrella hpat mutants lack cell-wall associated hydroxyproline arabinosides and can be rescued with exogenous cellulose, while global expression profiling shows that cell wall-associated genes are severely misexpressed, implicating a defect in cell wall formation during tip growth. Our findings point to a major role for HPATs in influencing cell elongation during tip growth in plants.pt_PT
dc.description.sponsorshipGordon and Betty Moore Foundation grant: (GBMF 2550.01); US Department of Agriculture grant: (2015-67013-22823); National Institute of Food and Agriculture.pt_PT
dc.identifier.citationMacAlister, C. A., Ortiz-Ramírez, C., Becker, J. D., Feijó, J. A. and Lippman, Z. B. (2016), Hydroxyproline O-arabinosyltransferase mutants oppositely alter tip growth in Arabidopsis thaliana and Physcomitrella patens. Plant J, 85: 193–208. doi:10.1111/tpj.13079pt_PT
dc.identifier.doi10.1111/tpj.13079pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/712
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationPLANT developmental biology: discovering the ORIGINS of form
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1111/tpj.13079/abstractpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectArabidopsis thalianapt_PT
dc.subjectPhyscomitrella patenspt_PT
dc.subjectcell wallpt_PT
dc.subjecttip growthpt_PT
dc.subjectglycosylationpt_PT
dc.subjectarabinosylationpt_PT
dc.subjectdevelopmentpt_PT
dc.subjectextensinspt_PT
dc.subjectpollinationpt_PT
dc.subjectprotonemapt_PT
dc.titleHydroxyprolineO-arabinosyltransferase mutants oppositely alter tip growth inArabidopsis thalianaandPhyscomitrella patenspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePLANT developmental biology: discovering the ORIGINS of form
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/238640/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBEX-BCM%2F0376%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-PLA%2F4018%2F2012/PT
oaire.citation.endPage208pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage193pt_PT
oaire.citation.titlePlant Journalpt_PT
oaire.citation.volume85pt_PT
oaire.fundingStreamFP7
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication22723dae-ee18-4c64-9872-698163bf9943
relation.isProjectOfPublicationa6c8d6b9-6beb-4205-a0a5-7e57a0aaf99d
relation.isProjectOfPublication8869b2a9-f7d7-4481-9d6b-9c18f6408fb4
relation.isProjectOfPublication.latestForDiscovery22723dae-ee18-4c64-9872-698163bf9943

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