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Inhibition of glutamine synthetase by phosphinothricin leads to transcriptome reprograming in root nodules of Medicago truncatula

2012-07Journal article Restricted access
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dc.contributor.authorSeabra, Ana R
dc.contributor.authorPereira, Patrícia A
dc.contributor.authorBecker, Jörg D
dc.contributor.authorCarvalho, Helena G
dc.date.accessioned2016-10-19T16:20:15Z
dc.date.available2016-10-19T16:20:15Z
dc.date.issued2012-07
dc.description.abstractGlutamine synthetase (GS) is a vital enzyme for the assimilation of ammonia into amino acids in higher plants. In legumes, GS plays a crucial role in the assimilation of the ammonium released by nitrogen-fixing bacteria in root nodules, constituting an important metabolic knob controlling the nitrogen (N) assimilatory pathways. To identify new regulators of nodule metabolism, we profiled the transcriptome of Medicago truncatula nodules impaired in N assimilation by specifically inhibiting GS activity using phosphinothricin (PPT). Global transcript expression of nodules collected before and after PPT addition (4, 8, and 24 h) was assessed using Affymetrix M. truncatula GeneChip arrays. Hundreds of genes were regulated at the three time points, illustrating the dramatic alterations in cell metabolism that are imposed on the nodules upon GS inhibition. The data indicate that GS inhibition triggers a fast plant defense response, induces premature nodule senescence, and promotes loss of root nodule identity. Consecutive metabolic changes were identified at the three time points analyzed. The results point to a fast repression of asparagine synthesis and of the glycolytic pathway and to the synthesis of glutamate via reactions alternative to the GS/GOGAT cycle. Several genes potentially involved in the molecular surveillance for internal organic N availability are identified and a number of transporters potentially important for nodule functioning are pinpointed. The data provided by this study contributes to the mapping of regulatory and metabolic networks involved in root nodule functioning and highlight candidate modulators for functional analysis.pt_PT
dc.identifier.citationInhibition of Glutamine Synthetase by Phosphinothricin Leads to Transcriptome Reprograming in Root Nodules of Medicago truncatula Ana R. Seabra, Patrícia A. Pereira, Jörg D. Becker, and Helena G. Carvalho Molecular Plant-Microbe Interactions 2012 25:7, 976-992pt_PT
dc.identifier.doi10.1094/MPMI-12-11-0322pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/704
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Phytopathological Societypt_PT
dc.relationExpression profiling studies to specify the symbiotic genetic programme of the model legume Medicago truncatula
dc.relation.publisherversionhttp://apsjournals.apsnet.org/doi/abs/10.1094/MPMI-12-11-0322pt_PT
dc.subjectAminobutyratespt_PT
dc.subjectAmmonium Compoundspt_PT
dc.subjectEnzyme Inhibitorspt_PT
dc.subjectGene Expression Profilingpt_PT
dc.subjectGene Expression Regulation, Plantpt_PT
dc.subjectGlutamate-Ammonia Ligasept_PT
dc.subjectMedicago truncatulapt_PT
dc.subjectNitrogenpt_PT
dc.subjectNitrogen Fixationpt_PT
dc.subjectOligonucleotide Array Sequence Analysispt_PT
dc.subjectPlant Leavespt_PT
dc.subjectPlant Proteinspt_PT
dc.subjectPlants, Genetically Modifiedpt_PT
dc.subjectRNA, Plantpt_PT
dc.subjectRoot Nodules, Plantpt_PT
dc.subjectSinorhizobium melilotipt_PT
dc.subjectSymbiosispt_PT
dc.subjectTime Factorspt_PT
dc.subjectTranscriptomept_PT
dc.titleInhibition of glutamine synthetase by phosphinothricin leads to transcriptome reprograming in root nodules of Medicago truncatulapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleExpression profiling studies to specify the symbiotic genetic programme of the model legume Medicago truncatula
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-GPL%2F70592%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-AAM%2F099577%2F2008/PT
oaire.citation.endPage992pt_PT
oaire.citation.issue7pt_PT
oaire.citation.startPage976pt_PT
oaire.citation.titleMolecular Plant-Microbe Interactionspt_PT
oaire.citation.volume25pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876-PPCDTI
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationb4c8cc78-8600-4b33-8318-140b9a4fa827
relation.isProjectOfPublicationb256e66f-3120-454c-9ad5-8a01456747c3
relation.isProjectOfPublication.latestForDiscoveryb4c8cc78-8600-4b33-8318-140b9a4fa827

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