Polo-like kinase 4 controls centriole duplication but does not directly regulate cytokinesis

Holland, A. J.; Fachinetti, D.; Da Cruz, S.; Zhu, Q.; Vitre, B.; Lince-Faria, M.; Chen, D.; Parish, N.; Verma, I. M.; Bettencourt-Dias, M.; Cleveland, D. W.

Publication

Polo-like kinase 4 controls centriole duplication but does not directly regulate cytokinesis

2012-03-20Journal article

dc.contributor.author	Holland, A. J.
dc.contributor.author	Fachinetti, D.
dc.contributor.author	Da Cruz, S.
dc.contributor.author	Zhu, Q.
dc.contributor.author	Vitre, B.
dc.contributor.author	Lince-Faria, M.
dc.contributor.author	Chen, D.
dc.contributor.author	Parish, N.
dc.contributor.author	Verma, I. M.
dc.contributor.author	Bettencourt-Dias, M.
dc.contributor.author	Cleveland, D. W.
dc.date.accessioned	2016-06-09T11:39:19Z
dc.date.available	2016-06-09T11:39:19Z
dc.date.issued	2012-03-20
dc.description.abstract	Centrioles organize the centrosome, and accurate control of their number is critical for the maintenance of genomic integrity. Centrioles duplicate once per cell cycle, and duplication is coordinated by Polo-like kinase 4 (Plk4). We previously demonstrated that Plk4 accumulation is autoregulated by its own kinase activity. However, loss of heterozygosity of Plk4 in mouse embryonic fibroblasts has been proposed to cause cytokinesis failure as a primary event, leading to centrosome amplification and gross chromosomal abnormalities. Using targeted gene disruption, we show that human epithelial cells with one inactivated Plk4 allele undergo neither cytokinesis failure nor increase in centrosome amplification. Plk4 is shown to localize exclusively at the centrosome, with none in the spindle midbody. Substantial depletion of Plk4 by small interfering RNA leads to loss of centrioles and subsequent spindle defects that lead to a modest increase in the rate of cytokinesis failure. Therefore, Plk4 is a centriole-localized kinase that does not directly regulate cytokinesis.	pt_PT
dc.description.sponsorship	National Institutes of Health grant: (GM29513); Ludwig Institute for Cancer Research; Leukemia and Lymphoma Society Special Fellowship; European Molecular Biology Organization (EMBO) long-term fellowship; Human Frontier Science Program (HFSP) postdoctoral fellowship.	pt_PT
dc.identifier.citation	Andrew J. Holland, Daniele Fachinetti, Sandrine Da Cruz, Quan Zhu, Benjamin Vitre, Mariana Lince-Faria, Denaly Chen, Nicole Parish, Inder M. Verma, Monica Bettencourt-Dias, and Don W. Cleveland Polo-like kinase 4 controls centriole duplication but does not directly regulate cytokinesis Mol. Biol. Cell 2012 23:10 1838-1845; First Published on March 28, 2012; doi:10.1091/mbc.E11-12-1043	pt_PT
dc.identifier.doi	10.1091/mbc.E11-12-1043	pt_PT
dc.identifier.uri	http://hdl.handle.net/10400.7/634
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	The American Society for Cell Biology	pt_PT
dc.relation.publisherversion	http://www.molbiolcell.org/content/23/10/1838.long	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by-nc-sa/4.0/	pt_PT
dc.subject	Animals	pt_PT
dc.subject	Cell Line	pt_PT
dc.subject	Centrioles	pt_PT
dc.subject	Centrosome	pt_PT
dc.subject	Fibroblasts	pt_PT
dc.subject	Gene Knockdown Techniques	pt_PT
dc.subject	Heterozygote	pt_PT
dc.subject	Humans	pt_PT
dc.subject	Mice	pt_PT
dc.subject	Microtubules	pt_PT
dc.subject	Phenotype	pt_PT
dc.subject	Protein Transport	pt_PT
dc.subject	Protein-Serine-Threonine Kinases	pt_PT
dc.subject	RNA Interference	pt_PT
dc.subject	Spindle Apparatus	pt_PT
dc.subject	Time-Lapse Imaging	pt_PT
dc.subject	Cytokinesis	pt_PT
dc.title	Polo-like kinase 4 controls centriole duplication but does not directly regulate cytokinesis	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.citation.endPage	1845	pt_PT
oaire.citation.issue	10	pt_PT
oaire.citation.startPage	1838	pt_PT
oaire.citation.title	Molecular Biology of the Cell	pt_PT
oaire.citation.volume	23	pt_PT
rcaap.rights	restrictedAccess	pt_PT
rcaap.type	article	pt_PT