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Quorum sensing regulation in Erwinia carotovora affects development of Drosophila melanogaster infected larvae

2019-12Journal article Open access
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dc.contributor.authorVieira, Filipe J. D.
dc.contributor.authorP, Nadal-Jimenez
dc.contributor.authorTeixeira, Luis
dc.contributor.authorXavier, Karina Bivar
dc.date.accessioned2020-03-17T14:01:32Z
dc.date.available2020-03-17T14:01:32Z
dc.date.issued2019-12
dc.description.abstractMulti-host bacteria must rapidly adapt to drastic environmental changes, relying on integration of multiple stimuli for an optimal genetic response. Erwinia spp. are phytopathogens that cause soft-rot disease in plants. Erwinia carotovora Ecc15 is used as a model for bacterial oral-route infection in Drosophila melanogaster as it harbors a gene, the Erwinia virulence factor (Evf), which has been previously shown to be a major determinant for infection of D. melanogaster gut. However, the factors involved in regulation of evf expression are poorly understood. We investigated whether evf could be controlled by quorum sensing since, in the Erwinia genus, quorum sensing regulates pectolytic enzymes, the major virulence factors needed to infect plants. Here, we show that transcription of evf is positively regulated by quorum sensing in Ecc15 via the acyl-homoserine lactone (AHL) signal synthase ExpI, and the AHL receptors ExpR1 and ExpR2. Moreover, we demonstrate that the GacS/A two-component system is partially required for evf expression. We also show that the load of Ecc15 in the gut depends upon the quorum sensing-mediated regulation of evf. Furthermore, we demonstrate that larvae infected with Ecc15 suffer a developmental delay as a direct consequence of the regulation of evf via quorum sensing. Overall, our results show that Ecc15 relies on quorum sensing to control production of both pectolytic enzymes and Evf. This regulation influences the interaction of Ecc15 with its two known hosts, indicating that quorum sensing and GacS/A signaling systems may impact bacterial dissemination via insect vectors that feed on rotting plants.pt_PT
dc.description.versioninfo:eu-repo/semantics/submittedVersionpt_PT
dc.identifier.doi10.1101/2019.12.13.876318pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/941
dc.language.isoengpt_PT
dc.peerreviewednopt_PT
dc.relationFCTpt_PT
dc.titleQuorum sensing regulation in Erwinia carotovora affects development of Drosophila melanogaster infected larvaept_PT
dc.typejournal article
dspace.entity.typePublication
person.familyNameNadal-Jimenez
person.familyNameTeixeira
person.familyNameXavier
person.givenNamePol
person.givenNameLuis
person.givenNameKarina
person.identifier.ciencia-idD317-CBF8-5590
person.identifier.ciencia-id641F-7328-B863
person.identifier.orcid0000-0002-9883-0920
person.identifier.orcid0000-0001-8326-6645
person.identifier.orcid0000-0001-5210-1434
person.identifier.ridA-7089-2018
person.identifier.ridH-6106-2013
person.identifier.ridB-9425-2008
person.identifier.scopus-author-id12777868200
person.identifier.scopus-author-id56304527800
person.identifier.scopus-author-id6603926993
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication8a785f4d-553f-4278-a0dc-df82bb8253fb
relation.isAuthorOfPublication23ec27dd-f98e-4b20-ab37-2e1717d04623
relation.isAuthorOfPublication26248334-983e-47c9-b1af-769585eec81b
relation.isAuthorOfPublication.latestForDiscovery8a785f4d-553f-4278-a0dc-df82bb8253fb

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