Repository logo
 
Publication

The Sinorhizobium meliloti EmrR Regulator Is Required for Efficient Colonization of Medicago sativa Root Nodules

2014-04Journal article Restricted access
Simple item page
dc.contributor.authorSantos, Mário R.
dc.contributor.authorMarques, Andreia T.
dc.contributor.authorBecker, Jörg D.
dc.contributor.authorMoreira, Leonilde M.
dc.date.accessioned2016-11-15T18:35:26Z
dc.date.available2016-11-15T18:35:26Z
dc.date.issued2014-04
dc.description.abstractThe nitrogen-fixing bacterium Sinorhizobium meliloti must adapt to diverse conditions encountered during its symbiosis with leguminous plants. We characterized a new symbiotically relevant gene, emrR (SMc03169), whose product belongs to the TetR family of repressors and is divergently transcribed from emrAB genes encoding a putative major facilitator superfamily-type efflux pump. An emrR deletion mutant produced more succinoglycan, displayed increased cell-wall permeability, and exhibited higher tolerance to heat shock. It also showed lower tolerance to acidic conditions, a reduced production of siderophores, and lower motility and biofilm formation. The simultaneous deletion of emrA and emrR genes restored the mentioned traits to the wild-type phenotype, except for survival under heat shock, which was lower than that displayed by the wild-type strain. Furthermore, the ΔemrR mutant as well as the double ΔemrAR mutant was impaired in symbiosis with Medicago sativa; it formed fewer nodules and competed poorly with the wild-type strain for nodule colonization. Expression profiling of the ΔemrR mutant showed decreased expression of genes involved in Nod-factor and rhizobactin biosynthesis and in stress responses. Expression of genes directing the biosynthesis of succinoglycan and other polysaccharides were increased. EmrR may therefore be involved in a regulatory network targeting membrane and cell wall modifications in preparation for colonization of root hairs during symbiosis.pt_PT
dc.description.sponsorshipFEDER; Fundação para a Ciência e a Tecnologia doctoral grant.pt_PT
dc.identifier.citationThe Sinorhizobium meliloti EmrR Regulator Is Required for Efficient Colonization of Medicago sativa Root Nodules Mário R. Santos, Andreia T. Marques, Jörg D. Becker, and Leonilde M. Moreira Molecular Plant-Microbe Interactions 2014 27:4, 388-399pt_PT
dc.identifier.doi10.1094/MPMI-09-13-0284-Rpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/711
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Phytopathological Societypt_PT
dc.relation.publisherversionhttp://apsjournals.apsnet.org/doi/abs/10.1094/MPMI-09-13-0284-Rpt_PT
dc.subjectAmino Acid Sequencept_PT
dc.subjectBacterial Adhesionpt_PT
dc.subjectBacterial Proteinspt_PT
dc.subjectBiofilmspt_PT
dc.subjectGene Deletionpt_PT
dc.subjectGene Expression Regulation, Bacterialpt_PT
dc.subjectMedicago sativapt_PT
dc.subjectMovementpt_PT
dc.subjectPlant Root Nodulationpt_PT
dc.subjectSinorhizobium melilotipt_PT
dc.titleThe Sinorhizobium meliloti EmrR Regulator Is Required for Efficient Colonization of Medicago sativa Root Nodulespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-MIC%2F113733%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0023%2F2013/PT
oaire.citation.endPage399pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage388pt_PT
oaire.citation.titleMolecular Plant-Microbe Interactionspt_PT
oaire.citation.volume27pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication737c72bf-4a3f-437f-b7c7-a0e0d72d4add
relation.isProjectOfPublication5b1f133c-c54b-4e4c-af57-b94829fdec77
relation.isProjectOfPublication.latestForDiscovery5b1f133c-c54b-4e4c-af57-b94829fdec77

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
mpmi-09-13-0284-r.pdf
Size:
1.31 MB
Format:
Adobe Portable Document Format
Description:
artigo principal
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

PG - Artigos