dc.contributor.author	Proença, João T.
dc.contributor.author	Barral, Duarte C.
dc.contributor.author	Gordo, Isabel
dc.date.accessioned	2017-07-12T14:13:37Z
dc.date.available	2017-07-12T14:13:37Z
dc.date.issued	2017-07-03
dc.description	There are no funders and sponsors indicated explicitly in the document. This deposit is composed by the main article plus the supplementary materials of the publication.	pt_PT
dc.description.abstract	Escherichia coli is both a harmless commensal in the intestines of many mammals, as well as a dangerous pathogen. The evolutionary paths taken by strains of this species in the commensal-to-pathogen transition are complex and can involve changes both in the core genome, as well in the pan-genome. One way to understand the likely paths that a commensal strain of E. coli takes when evolving pathogenicity is through experimentally evolving the strain under the selective pressures that it will have to withstand as a pathogen. Here, we report that a commensal strain, under continuous pressure from macrophages, recurrently acquired a transposable element insertion, which resulted in two key phenotypic changes: increased intracellular survival, through the delay of phagosome maturation and increased ability to escape macrophages. We further show that the acquisition of the pathoadaptive traits was accompanied by small but significant changes in the transcriptome of macrophages upon infection. These results show that under constant pressures from a key component of the host immune system, namely macrophage phagocytosis, commensal E. coli rapidly acquires pathoadaptive mutations that cause transcriptome changes associated to the host-microbe duet.	pt_PT
dc.description.sponsorship	There are no funders and sponsors indicated explicitly in the document.	pt_PT
dc.description.version	info:eu-repo/semantics/publishedVersion	pt_PT
dc.identifier.citation	Proença, J. T., Barral, D. C. & Gordo, I. Commensal-to-pathogen transition: One-single transposon insertion results in two pathoadaptive traits in Escherichia coli -macrophage interaction. Sci Rep 7, 4504 (2017). doi:10.1038/s41598-017-04081-1	pt_PT
dc.identifier.doi	10.1038/s41598-017-04081-1	pt_PT
dc.identifier.uri	http://hdl.handle.net/10400.7/774
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	Nature Publishing Group	pt_PT
dc.relation	There are no funders and sponsors indicated explicitly in the document.	pt_PT
dc.relation.publisherversion	https://www.nature.com/articles/s41598-017-04081-1	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	pt_PT
dc.subject	Bacterial genetics	pt_PT
dc.subject	Experimental evolution	pt_PT
dc.subject	Infection	pt_PT
dc.title	Commensal-to-pathogen transition: One-single transposon insertion results in two pathoadaptive traits in Escherichia coli -macrophage interaction	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.citation.endPage	12	pt_PT
oaire.citation.issue	1	pt_PT
oaire.citation.startPage	1	pt_PT
oaire.citation.title	Scientific Reports	pt_PT
oaire.citation.volume	7	pt_PT
rcaap.rights	openAccess	pt_PT
rcaap.type	article	pt_PT

Commensal-to-pathogen transition: One-single transposon insertion results in two pathoadaptive traits in Escherichia coli -macrophage interaction

Files

Original bundle

License bundle

Collections