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Intravital placenta imaging reveals microcirculatory dynamics impact on sequestration and phagocytosis of Plasmodium-infected erythrocytes

2013Journal article Open access
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dc.contributor.authorMoraes, Luciana Vieira de
dc.contributor.authorTadokoro, Carlos Eduardo
dc.contributor.authorGómez-Conde, Ivan
dc.contributor.authorOlivieri, David N.
dc.contributor.authorPenha-Gonçalves, Carlos
dc.date.accessioned2013-07-31T09:15:42Z
dc.date.available2013-07-31T09:15:42Z
dc.date.issued2013
dc.description.abstractMalaria in pregnancy is exquisitely aggressive, causing a range of adverse maternal and fetal outcomes prominently linked to Plasmodium-infected erythrocyte cytoadherence to fetal trophoblast. To elucidate the physiopathology of infected erythrocytes (IE) sequestration in the placenta we devised an experimental system for intravital placental examination of P. berghei-infected mice. BALB/c females were mated to C57Bl/6 CFP+ male mice and infected with GFP+ P. berghei IE, and at gestational day 18, placentas were exposed for time-lapse imaging acquisition under two-photon microscopy. Real-time images and quantitative measurements revealed that trophoblast conformational changes transiently restrain blood flow in the mouse placental labyrinth. The complex dynamics of placental microcirculation promotes IE accumulation in maternal blood spaces with low blood flow and allows the establishment of stable IE-trophoblast contacts. Further, we show that the fate of sequestered IE includes engulfment by both macrophagic and trophoblastic fetal-derived cells. These findings reinforce the current paradigm that IE interact with the trophoblast and provide definitive evidence on two novel pathogenesis mechanisms: (1) trophoblast layer controls placental microcirculation promoting IE sequestration; and (2) fetal-derived placental cells engulf sequestered IE.por
dc.identifier.citationMoraes, L. V. de, Tadokoro, C. E., Gómez-Conde, I., Olivieri, D. N., & Penha-Gonçalves, C. (2013). Intravital placenta imaging reveals microcirculatory dynamics impact on sequestration and phagocytosis of Plasmodium-infected erythrocytes. PLoS pathogens, 9(1), e1003154.
dc.identifier.issn1553-7374
dc.identifier.urihttp://hdl.handle.net/10400.7/245
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherPublic Library of Sciencepor
dc.relation.publisherversionhttp://dx.doi.org/10.1371/journal.ppat.1003154por
dc.subjectChondroitin-Sulfate-Apor
dc.subjectFalciparum-Malariapor
dc.subjectMouse Placentapor
dc.subjectBlood-Flowpor
dc.subjectPregnancypor
dc.subjectBergheipor
dc.subjectSyncytiotrophoblastpor
dc.subjectPathologypor
dc.titleIntravital placenta imaging reveals microcirculatory dynamics impact on sequestration and phagocytosis of Plasmodium-infected erythrocytespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPagee1003154por
oaire.citation.titlePLOS Pathogenspor
oaire.citation.volume9(1)por
rcaap.rightsopenAccesspor
rcaap.typearticlepor

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