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Potential Mechanisms Underlying Response to Effects of the Fungicide Pyrimethanil from Gene Expression Profiling inSaccharomyces cerevisiae

2014-06-11Journal article Restricted access
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dc.contributor.authorGil, Fátima N.
dc.contributor.authorBecker, Jörg D.
dc.contributor.authorViegas, Cristina A.
dc.date.accessioned2016-11-22T16:08:53Z
dc.date.available2016-11-22T16:08:53Z
dc.date.issued2014-06-11
dc.description.abstractPyrimethanil is a fungicide mostly applied in vineyards. When misused, residue levels detected in grape must or in the environment may be of concern. The present work aimed to analyze mechanisms underlying response to deleterious effects of pyrimethanil in the eukaryotic model Saccharomyces cerevisiae. Pyrimethanil concentration-dependent effects at phenotypic (inhibition of growth) and transcriptomic levels were examined. For transcriptional profiling, analysis focused on two sublethal exposure conditions that inhibited yeast growth by 20% or 50% compared with control cells not exposed to the fungicide. Gene expression modifications increased with the magnitude of growth inhibition, in numbers and fold-change of differentially expressed genes and in diversity of over-represented functional categories. These included mostly biosynthesis of arginine and sulfur amino acids metabolism, as well as energy conservation, antioxidant response, and multidrug transport. Several pyrimethanil-responsive genes encoded proteins sharing significant homology with proteins from phytopathogenic fungi and ecologically relevant higher eukaryotes.pt_PT
dc.description.sponsorshipEuropean Funds for Regional Development : (FEDER through the Competitiveness Factors Operational Programme COMPETE); Fundação para a Ciência e Tecnologia grant: (PEst-OE/EQB/LA0023/2011).pt_PT
dc.identifier.citationPotential Mechanisms Underlying Response to Effects of the Fungicide Pyrimethanil from Gene Expression Profiling in Saccharomyces cerevisiae Fátima N. Gil, Jörg D. Becker, and Cristina A. Viegas Journal of Agricultural and Food Chemistry 2014 62 (23), 5237-5247 DOI: 10.1021/jf5007775pt_PT
dc.identifier.doi10.1021/jf5007775pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/720
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Chemical Societypt_PT
dc.relationTerbAzineBiorem - Studies on the efficacy and scale-up of bioremediation strategies for soils contaminated with the herbicide terbuthylazine.
dc.relationIDENTIFICATION OF MOLECULAR BIOMARKERS RELEVANT FOR ENVIRONMENTAL BIOMONITORING OF PESTICIDE TOXICITY USING SIMPLE EUKARYOTIC MODELS
dc.relation.publisherversionhttp://pubs.acs.org/doi/abs/10.1021/jf5007775pt_PT
dc.subjectpyrimethanilpt_PT
dc.subjectsublethal effectspt_PT
dc.subjecttoxicogenomicspt_PT
dc.subjecttoxicity mechanismspt_PT
dc.subjectmode of actionpt_PT
dc.subjectSaccharomyces cerevisiaept_PT
dc.titlePotential Mechanisms Underlying Response to Effects of the Fungicide Pyrimethanil from Gene Expression Profiling inSaccharomyces cerevisiaept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTerbAzineBiorem - Studies on the efficacy and scale-up of bioremediation strategies for soils contaminated with the herbicide terbuthylazine.
oaire.awardTitleIDENTIFICATION OF MOLECULAR BIOMARKERS RELEVANT FOR ENVIRONMENTAL BIOMONITORING OF PESTICIDE TOXICITY USING SIMPLE EUKARYOTIC MODELS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAMB%2F64230%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAAC-AMB%2F111317%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F60933%2F2009/PT
oaire.citation.endPage5247pt_PT
oaire.citation.issue23pt_PT
oaire.citation.startPage5237pt_PT
oaire.citation.titleJournal of Agricultural and Food Chemistrypt_PT
oaire.citation.volume62pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationfc86bd6d-a572-4c39-a570-bc49882b05b2
relation.isProjectOfPublication36db6520-6b8e-4117-bc38-28c8f7e9e90d
relation.isProjectOfPublication90b611cd-2b1d-43da-84a9-8d399244318a
relation.isProjectOfPublication.latestForDiscoveryfc86bd6d-a572-4c39-a570-bc49882b05b2

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