Publication
The multigenic structure of the MHC locus contributes to positive selection efficiency: A role for MHC class II gene-genespecific
| dc.contributor.author | Monteiro, Miguel Caetano | |
| dc.contributor.author | Couceiro, Sofia | |
| dc.contributor.author | Penha-Gonçalves, Carlos | |
| dc.date.accessioned | 2010-05-14T11:15:31Z | |
| dc.date.available | 2010-05-14T11:15:31Z | |
| dc.date.issued | 2005 | |
| dc.description | This deposit is in restrictedAccess (it can't be in open access to the public), and can only be accessed by two ways: either by requesting a legal copy from the author (the email contact present in this deposit) or by visiting the following link: https://onlinelibrary.wiley.com/doi/abs/10.1002/eji.200535190 | pt_PT |
| dc.description.abstract | The study of T cell positive selection in the thymus has long been focused on the specificity of the MHC-TCR interactions, making use of genetically manipulated mice that display TCR specificities or selecting peptides of limited diversity. However, little is known on the role of the MHC molecules irrespective of the peptide specificity and the implications of MHC multigenic structure in thymic positive selection have not been addressed. Here, we investigated the effect of MHC class II genetic configuration on the positive selection efficiency of naturally generated pre-selection repertoires in the mouse thymus. Analysis of positively selected thymocyte populations in MHC-congenic and -transgenic mice revealed that expression of I-E molecule in the thymic cortex increases positive selection efficiency of CD4 cells by approximately 50%.We show that increments in positive selection attributable to either the I-A and I-E genes are not due to increased MHC class II expression in the thymic cortex and are not affected by the number of MHC alleles. Collectively, our findings imply that MHC class II generestricted TCR specificities significantly contribute to positive selection efficiency, introducing the notion that multigenic structure of the MHC locus serves to increase selection of non-overlapping TCR repertoires. | pt |
| dc.identifier.citation | Monteiro, M. C., Couceiro S., Penha-Gonçalves C. (2013). The multigenic structure of the MHC locus contributes to positive selection efficiency: a role for MHC class II gene-specific restriction. European Journal of Immunology. 35(12): 1-10 | pt |
| dc.identifier.issn | 1521-4141 | |
| dc.identifier.uri | http://hdl.handle.net/10400.7/114 | |
| dc.language.iso | eng | |
| dc.peerreviewed | Yes | |
| dc.publisher | Wiley | pt |
| dc.relation.publisherversion | http://dx.doi.org/10.1002/eji.200535190 | |
| dc.subject | MHC | pt |
| dc.subject | Multigenic | pt |
| dc.subject | Repertoire development | pt |
| dc.subject | Thymic selection | pt |
| dc.title | The multigenic structure of the MHC locus contributes to positive selection efficiency: A role for MHC class II gene-genespecific | pt |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 3630 | pt |
| oaire.citation.startPage | 3622 | pt |
| oaire.citation.title | European Journal of Immunology | pt |
| rcaap.rights | restrictedAccess | pt |
| rcaap.type | article | pt |