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Dynamics of Histone H3 Deposition In Vivo Reveal a Nucleosome Gap-Filling Mechanism for H3.3 to Maintain Chromatin Integrity

2011-12-23Journal article Open access
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dc.contributor.authorRay-Gallet, Dominique
dc.contributor.authorWoolfe, Adam
dc.contributor.authorVassias, Isabelle
dc.contributor.authorPellentz, Céline
dc.contributor.authorLacoste, Nicolas
dc.contributor.authorPuri, Aastha
dc.contributor.authorSchultz, David C.
dc.contributor.authorPchelintsev, Nikolay A.
dc.contributor.authorAdams, Peter D.
dc.contributor.authorJansen, Lars E.T.
dc.contributor.authorAlmouzni, Geneviève
dc.date.accessioned2017-11-15T12:24:37Z
dc.date.available2017-11-15T12:24:37Z
dc.date.issued2011-12-23
dc.descriptionThe deposited article is a post-print version and has peer review. The deposited article version contains attached the supplementary materials within the pdf. This publication hasn't any creative commons license associated.pt_PT
dc.description.abstractEstablishment of a proper chromatin landscape is central to genome function. Here, we explain H3 variant distribution by specific targeting and dynamics of deposition involving the CAF-1 and HIRA histone chaperones. Impairing replicative H3.1 incorporation via CAF-1 enables an alternative H3.3 deposition at replication sites via HIRA. Conversely, the H3.3 incorporation throughout the cell cycle via HIRA cannot be replaced by H3.1. ChIP-seq analyses reveal correlation between HIRA-dependent H3.3 accumulation and RNA pol II at transcription sites and specific regulatory elements, further supported by their biochemical association. The HIRA complex shows unique DNA binding properties, and depletion of HIRA increases DNA sensitivity to nucleases. We propose that protective nucleosome gap filling of naked DNA by HIRA leads to a broad distribution of H3.3, and HIRA association with Pol II ensures local H3.3 enrichment at specific sites. We discuss the importance of this H3.3 deposition as a salvage pathway to maintain chromatin integrity.pt_PT
dc.description.sponsorshipLigue Nationale contre le Cancer (Equipe labellisée Ligue 2010); PIC Programs; the European Commission Network of Excellence EpiGeneSys (HEALTH-F4-2010-257082), the European Commission ITN FP7-PEOPLE-2007-215148 “Image DDR” and FP7-PEOPLE-2008-238176 “Nucleosome 4D,” ERC Advanced Grant 2009-AdG_20090506 “Eccentric,” the European Commission large-scale integrating project FP7_HEALTH-2010-259743 “MODHEP,” ANR “ECenS” ANR-09-BLAN-0257-01, ANR “ChromaTin” ANR-10-BLAN-1326-03, and INCa “GepiG.” The lab of P.D.A. is funded by NIA program project P01 AG031862. L.E.T.J. was supported by the Fundação Calouste Gulbenkian, Fundação para a Ciência e a Tecnologia (FCT) grant BIA-PRO/100537/2008, the European Commission FP7 programme, and an EMBO installation grant.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationDominique Ray-Gallet, Adam Woolfe, Isabelle Vassias, Céline Pellentz, Nicolas Lacoste, Aastha Puri, David C. Schultz, Nikolay A. Pchelintsev, Peter D. Adams, Lars E.T. Jansen, Geneviève Almouzni, Dynamics of Histone H3 Deposition In Vivo Reveal a Nucleosome Gap-Filling Mechanism for H3.3 to Maintain Chromatin Integrity, Molecular Cell, Volume 44, Issue 6, 23 December 2011, Pages 928-941, ISSN 1097-2765, https://doi.org/10.1016/j.molcel.2011.12.006. (https://www.sciencedirect.com/science/article/pii/S1097276511009452)pt_PT
dc.identifier.doi10.1016/j.molcel.2011.12.006pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/806
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherCell Presspt_PT
dc.relationERC Advanced Grant 2009-AdG_20090506pt_PT
dc.relationEpigenetics towards systems biology
dc.relationHigh Resolution Microscopy in the DNA Damage Response
dc.relationNucleosome Structure & Function across Biological Scales and Biological Function
dc.relationINCa “GepiG.”pt_PT
dc.relationSystems biology of liver cancer: an integrative genomic-epigenomic approach
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S1097276511009452pt_PT
dc.subjectCell Cycle Proteinspt_PT
dc.subjectChromatin Assembly Factor-1pt_PT
dc.subjectDNA Replicationpt_PT
dc.subjectDeoxyribonucleasespt_PT
dc.subjectHeLa Cellspt_PT
dc.subjectHistone Chaperonespt_PT
dc.subjectHistonespt_PT
dc.subjectHumanspt_PT
dc.subjectMolecular Chaperonespt_PT
dc.subjectNucleosomespt_PT
dc.subjectRNA Polymerase IIpt_PT
dc.subjectTranscription Factorspt_PT
dc.titleDynamics of Histone H3 Deposition In Vivo Reveal a Nucleosome Gap-Filling Mechanism for H3.3 to Maintain Chromatin Integritypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEpigenetics towards systems biology
oaire.awardTitleHigh Resolution Microscopy in the DNA Damage Response
oaire.awardTitleNucleosome Structure & Function across Biological Scales and Biological Function
oaire.awardTitleSystems biology of liver cancer: an integrative genomic-epigenomic approach
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/257082/EU
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/215148/EU
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/238176/EU
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/259743/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-PRO%2F100537%2F2008/PT
oaire.citation.endPage941pt_PT
oaire.citation.issue6pt_PT
oaire.citation.startPage928pt_PT
oaire.citation.titleMolecular Cellpt_PT
oaire.citation.volume44pt_PT
oaire.fundingStreamFP7
oaire.fundingStreamFP7
oaire.fundingStreamFP7
oaire.fundingStreamFP7
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100008530
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project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameEuropean Commission
project.funder.nameEuropean Commission
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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