Publication
Pericentrin-mediated SAS-6 recruitment promotes centriole assembly
| dc.contributor.author | Ito, Daisuke | |
| dc.contributor.author | Zitouni, Sihem | |
| dc.contributor.author | Jana, Swadhin Chandra | |
| dc.contributor.author | Duarte, Paulo | |
| dc.contributor.author | Surkont, Jaroslaw | |
| dc.contributor.author | Carvalho-Santos, Zita | |
| dc.contributor.author | Pereira-Leal, José B | |
| dc.contributor.author | Ferreira, Miguel Godinho | |
| dc.contributor.author | Bettencourt-Dias, Mónica | |
| dc.date.accessioned | 2019-07-25T13:51:06Z | |
| dc.date.available | 2019-07-25T13:51:06Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | The centrosome is composed of two centrioles surrounded by a microtubule-nucleating pericentriolar material (PCM). Although centrioles are known to regulate PCM assembly, it is less known whether and how the PCM contributes to centriole assembly. Here we investigate the interaction between centriole components and the PCM by taking advantage of fission yeast, which has a centriole-free, PCM-containing centrosome, the SPB. Surprisingly, we observed that several ectopically-expressed animal centriole components such as SAS-6 are recruited to the SPB. We revealed that a conserved PCM component, Pcp1/pericentrin, interacts with and recruits SAS-6. This interaction is conserved and important for centriole assembly, particularly its elongation. We further explored how yeasts kept this interaction even after centriole loss and showed that the conserved calmodulin-binding region of Pcp1/pericentrin is critical for SAS-6 interaction. Our work suggests that the PCM not only recruits and concentrates microtubule-nucleators, but also the centriole assembly machinery, promoting biogenesis close by. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.doi | 10.7554/eLife.41418 | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.7/899 | |
| dc.language.iso | eng | pt_PT |
| dc.title | Pericentrin-mediated SAS-6 recruitment promotes centriole assembly | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.volume | 8 | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |