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Heat and water stress induce unique transcriptional signatures of heat-shock proteins and transcription factors in grapevine

2013-10-12Journal article Open access
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dc.contributor.authorRocheta, Margarida
dc.contributor.authorBecker, Jörg D.
dc.contributor.authorCoito, João L.
dc.contributor.authorCarvalho, Luísa
dc.contributor.authorAmâncio, Sara
dc.date.accessioned2016-11-16T18:24:44Z
dc.date.available2016-11-16T18:24:44Z
dc.date.issued2013-10-12
dc.description.abstractGrapevine is an extremely important crop worldwide.In southern Europe, post-flowering phases of the growth cycle can occur under high temperatures, excessive light, and drought conditions at soil and/or atmospheric level. In this study, we subjected greenhouse grown grapevine, variety Aragonez, to two individual abiotic stresses, water deficit stress(WDS), and heat stress (HS). The adaptation of plants to stress is a complex response triggered by cascades of molecular net works involved in stress perception, signal transduction, and the expression of specific stress-related genes and metabolites. Approaches such as array-based transcript profiling allow assessing the expression of thousands of genes in control and stress tissues. Using microarrays, we analyzed the leaf transcriptomic profile of the grapevine plants. Photosynthesis measurements verified that the plants were significantly affected by the stresses applied. Leaf gene expression was obtained using a high-throughput transcriptomic grapevine array, the 23K custom-made Affymetrix Vitis GeneChip. We identified 1,594 genes as differentially expressed between control and treatments and grouped them into ten major functional categories using MapMan software. The transcriptome of Aragonez was more significantly affected by HS when compared with WDS. The number of genes coding for heat-shock proteins and transcription factors expressed solely in response to HS suggesting their expression as unique signatures of HS. However, across-talk between the response pathways to both stresses was observed at the level of AP2/ERF transcription factors.pt_PT
dc.description.sponsorshipERA-NET Plant Genomics 006/2006.pt_PT
dc.identifier.citationRocheta, M., Becker, J.D., Coito, J.L. et al. Funct Integr Genomics (2014) 14: 135. doi:10.1007/s10142-013-0338-zpt_PT
dc.identifier.doi10.1007/s10142-013-0338-zpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/715
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Verlagpt_PT
dc.relationVITIMULTISTRESS - Gene expression in grapevine (Vitis vinifera L) under multiple abiotic stresses.
dc.relationGENES THAT CONTROL SEX DETERMINATION AND EVOLUTION OF MONOEICY IN GRAPEVINE: THE ROLE OF FLOWER MERISTEM AND ORGAN IDENTITY GENES
dc.relation.publisherversionhttp://link.springer.com/article/10.1007%2Fs10142-013-0338-zpt_PT
dc.subjectCluster Analysispt_PT
dc.subjectDehydrationpt_PT
dc.subjectHeat-Shock Proteinspt_PT
dc.subjectOligonucleotide Array Sequence Analysispt_PT
dc.subjectPlant Leavespt_PT
dc.subjectPlant Proteinspt_PT
dc.subjectStress, Physiologicalpt_PT
dc.subjectTranscription Factorspt_PT
dc.subjectTranscriptomept_PT
dc.subjectVitispt_PT
dc.subjectGene Expression Regulation, Plantpt_PT
dc.titleHeat and water stress induce unique transcriptional signatures of heat-shock proteins and transcription factors in grapevinept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleVITIMULTISTRESS - Gene expression in grapevine (Vitis vinifera L) under multiple abiotic stresses.
oaire.awardTitleGENES THAT CONTROL SEX DETERMINATION AND EVOLUTION OF MONOEICY IN GRAPEVINE: THE ROLE OF FLOWER MERISTEM AND ORGAN IDENTITY GENES
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-GPL%2F099624%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FAGR%2FUI0240%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F64905%2F2009/PT
oaire.citation.endPage148pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage135pt_PT
oaire.citation.titleFunctional and Integrative Genomicspt_PT
oaire.citation.volume14pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationbd579e29-17ab-41cf-bc75-82b4a3cf814d
relation.isProjectOfPublicationf38fb6e0-f54c-4ec9-8425-71e072da8741
relation.isProjectOfPublicationc87293d9-3313-4a7e-8706-b9a01a87b9f9
relation.isProjectOfPublication.latestForDiscoveryc87293d9-3313-4a7e-8706-b9a01a87b9f9

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