A Discrete Model of Drosophila Eggshell Patterning Reveals Cell-Autonomous and Juxtacrine Effects

Fauré, Adrien; Vreede, Barbara M. I.; Sucena, Élio; Chaouiya, Claudine

Publication

A Discrete Model of Drosophila Eggshell Patterning Reveals Cell-Autonomous and Juxtacrine Effects

2014-03-27Journal article

dc.contributor.author	Fauré, Adrien
dc.contributor.author	Vreede, Barbara M. I.
dc.contributor.author	Sucena, Élio
dc.contributor.author	Chaouiya, Claudine
dc.date.accessioned	2015-10-01T16:32:55Z
dc.date.available	2015-10-01T16:32:55Z
dc.date.issued	2014-03-27
dc.description.abstract	The Drosophila eggshell constitutes a remarkable system for the study of epithelial patterning, both experimentally and through computational modeling. Dorsal eggshell appendages arise from specific regions in the anterior follicular epithelium that covers the oocyte: two groups of cells expressing broad (roof cells) bordered by rhomboid expressing cells (floor cells). Despite the large number of genes known to participate in defining these domains and the important modeling efforts put into this developmental system, key patterning events still lack a proper mechanistic understanding and/or genetic basis, and the literature appears to conflict on some crucial points. We tackle these issues with an original, discrete framework that considers single-cell models that are integrated to construct epithelial models. We first build a phenomenological model that reproduces wild type follicular epithelial patterns, confirming EGF and BMP signaling input as sufficient to establish the major features of this patterning system within the anterior domain. Importantly, this simple model predicts an instructive juxtacrine signal linking the roof and floor domains. To explore this prediction, we define a mechanistic model that integrates the combined effects of cellular genetic networks, cell communication and network adjustment through developmental events. Moreover, we focus on the anterior competence region, and postulate that early BMP signaling participates with early EGF signaling in its specification. This model accurately simulates wild type pattern formation and is able to reproduce, with unprecedented level of precision and completeness, various published gain-of-function and loss-of-function experiments, including perturbations of the BMP pathway previously seen as conflicting results. The result is a coherent model built upon rules that may be generalized to other epithelia and developmental systems.	pt_PT
dc.description.sponsorship	FCT fellowship: SFRH/BD/33216/2007, Fundação Calouste Gulbenkian/Instituto Gulbenkian de Ciência.	pt_PT
dc.identifier	10.1371/journal.pcbi.1003527
dc.identifier.citation	Fauré A, Vreede BMI, Sucena É, Chaouiya C (2014) A Discrete Model of Drosophila Eggshell Patterning Reveals Cell-Autonomous and Juxtacrine Effects. PLoS Comput Biol 10(3): e1003527. doi:10.1371/journal.pcbi.1003527	pt_PT
dc.identifier.doi	10.1371/journal.pcbi.1003527
dc.identifier.doi	10.1371/journal.pcbi.1003527
dc.identifier.doi	10.1371/journal.pcbi.1003527
dc.identifier.uri	http://hdl.handle.net/10400.7/338
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	PLOS	pt_PT
dc.relation	MALIN: Modular modelling and Analysis of Large biological Interacting Networks
dc.relation.publisherversion	http://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1003527	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	pt_PT
dc.subject	Drosophila melanogaster	pt_PT
dc.subject	genetic	pt_PT
dc.title	A Discrete Model of Drosophila Eggshell Patterning Reveals Cell-Autonomous and Juxtacrine Effects	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.awardTitle	MALIN: Modular modelling and Analysis of Large biological Interacting Networks
oaire.awardURI	info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEIA-CCO%2F099229%2F2008/PT
oaire.citation.endPage	15	pt_PT
oaire.citation.issue	3	pt_PT
oaire.citation.startPage	1	pt_PT
oaire.citation.title	PLOS Computational Biology	pt_PT
oaire.citation.volume	10	pt_PT
oaire.fundingStream	3599-PPCDT
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.name	Fundação para a Ciência e a Tecnologia
rcaap.rights	openAccess	pt_PT
rcaap.type	article	pt_PT
relation.isProjectOfPublication	820042aa-3860-49b5-9f01-646a11e76442
relation.isProjectOfPublication.latestForDiscovery	820042aa-3860-49b5-9f01-646a11e76442