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PLK4 trans-Autoactivation Controls Centriole Biogenesis in Space

2015-10-26Journal article Open access
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dc.contributor.authorLopes, Carla A.M.
dc.contributor.authorJana, Swadhin Chandra
dc.contributor.authorCunha-Ferreira, Inês
dc.contributor.authorZitouni, Sihem
dc.contributor.authorBento, Inês
dc.contributor.authorDuarte, Paulo
dc.contributor.authorGilberto, Samuel
dc.contributor.authorFreixo, Francisco
dc.contributor.authorGuerrero, Adán
dc.contributor.authorFrancia, Maria
dc.contributor.authorLince-Faria, Mariana
dc.contributor.authorCarneiro, Jorge
dc.contributor.authorBettencourt-Dias, Mónica
dc.date.accessioned2018-02-07T12:47:34Z
dc.date.available2018-02-07T12:47:34Z
dc.date.issued2015-10-26
dc.descriptionThe deposited article is a post-print version and has been submitted to peer review.pt_PT
dc.descriptionThis publication hasn't any creative commons license associated.pt_PT
dc.descriptionThe deposited article version contains attached the supplementary materials within the pdf.pt_PT
dc.description.abstractCentrioles are essential for cilia and centrosome assembly. In centriole-containing cells, centrioles always form juxtaposed to pre-existing ones, motivating a century-old debate on centriole biogenesis control. Here, we show that trans-autoactivation of Polo-like kinase 4 (PLK4), the trigger of centriole biogenesis, is a critical event in the spatial control of that process. We demonstrate that centrioles promote PLK4 activation through its recruitment and local accumulation. Though centriole removal reduces the proportion of active PLK4, this is rescued by concentrating PLK4 to the peroxisome lumen. Moreover, while mild overexpression of PLK4 only triggers centriole amplification at the existing centriole, higher PLK4 levels trigger both centriolar and cytoplasmatic (de novo) biogenesis. Hence, centrioles promote their assembly locally and disfavor de novo synthesis. Similar mechanisms enforcing the local concentration and/or activity of other centriole components are likely to contribute to the spatial control of centriole biogenesis under physiological conditions.pt_PT
dc.description.sponsorshipFundação Portuguesa para a Ciência e Tecnologia grants: (SFRH/BPD/87479/2012, PTDC/BBB-BEP/1724/2012, HMSP-CT/SAU-ICT/0075/2009, PTDC/SAU-OBD/105616/2008, EXPL/BIM-ONC/0830/2013, PTDC/BBB-BEP/1724/2012); EMBO installation grant; ERC starting grant: (PFE-GI-UE-ERC-2010-StG-261344).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCarla A.M. Lopes, Swadhin Chandra Jana, Inês Cunha-Ferreira, Sihem Zitouni, Inês Bento, Paulo Duarte, Samuel Gilberto, Francisco Freixo, Adán Guerrero, Maria Francia, Mariana Lince-Faria, Jorge Carneiro, Mónica Bettencourt-Dias, PLK4 trans-Autoactivation Controls Centriole Biogenesis in Space, Developmental Cell, Volume 35, Issue 2, 2015, Pages 222-235, ISSN 1534-5807, https://doi.org/10.1016/j.devcel.2015.09.020. (http://www.sciencedirect.com/science/article/pii/S1534580715006218)pt_PT
dc.identifier.doi10.1016/j.devcel.2015.09.020pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/830
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationControl of Centriole Structure And Number
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1534580715006218?via%3Dihub#!pt_PT
dc.subjectAnimalspt_PT
dc.subjectCentriolespt_PT
dc.subjectCentrosomept_PT
dc.subjectDrosophila Proteinspt_PT
dc.subjectDrosophila melanogasterpt_PT
dc.subjectGene Expression Regulation, Developmentalpt_PT
dc.subjectPeroxisomespt_PT
dc.subjectPhosphorylationpt_PT
dc.subjectProtein-Serine-Threonine Kinasespt_PT
dc.subjectSpermatogenesispt_PT
dc.titlePLK4 trans-Autoactivation Controls Centriole Biogenesis in Spacept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleControl of Centriole Structure And Number
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F87479%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/261344/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/HMSP-CT%2FSAU-ICT%2F0075%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-OBD%2F105616%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/EXPL%2FBIM-ONC%2F0830%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBBB-BEP%2F1724%2F2012/PT
oaire.citation.endPage235pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage222pt_PT
oaire.citation.titleDevelopmental Cellpt_PT
oaire.citation.volume35pt_PT
oaire.fundingStreamSFRH
oaire.fundingStreamFP7
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isProjectOfPublication.latestForDiscovery12b42d08-d406-470c-a972-2ac4992a9b3e

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