Intercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to sperm

Jiang, Hua; Yi, Jun; Boavida, Leonor C.; Chen, Yuan; Becker, Jörg D.; Köhler, Claudia; McCormick, Sheila

http://hdl.handle.net/10400.7/500

Use this identifier to reference this record.

Name:	Description:	Size:	Format:
PNAS-2015-Jiang-13378-83.pdf	artigo principal	1.07 MB	Adobe PDF	Download

Send Feedback

Authors

Abstract(s)

An Arabidopsis pollen grain (male gametophyte) consists of three cells: the vegetative cell, which forms the pollen tube, and two sperm cells enclosed within the vegetative cell. It is still unclear if there is intercellular communication between the vegetative cell and the sperm cells. Here we show that ABA-hypersensitive germination3 (AHG3), encoding a protein phosphatase, is specifically transcribed in the vegetative cell but predominantly translated in sperm cells. We used a series of deletion constructs and promoter exchanges to document transport of AHG3 transcripts from the vegetative cell to sperm and showed that their transport requires sequences in both the 5' UTR and the coding region. Thus, in addition its known role in transporting sperm during pollen tube growth, the vegetative cell also contributes transcripts to the sperm cells.

Keywords

pollen sperm mRNA transport protein phosphatase 2C vegetative nucleus

URI

http://hdl.handle.net/10400.7/500

Citation

Hua Jiang, Jun Yi, Leonor C. Boavida, Yuan Chen, Jörg D. Becker, Claudia Köhler, and Sheila McCormick Intercellular communication in Arabidopsis thaliana pollen discovered via AHG3 transcript movement from the vegetative cell to sperm PNAS 2015 112: 13378-13383.