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Intercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to sperm

2015-10-27Journal article Open access
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dc.contributor.authorJiang, Hua
dc.contributor.authorYi, Jun
dc.contributor.authorBoavida, Leonor C.
dc.contributor.authorChen, Yuan
dc.contributor.authorBecker, Jörg D.
dc.contributor.authorKöhler, Claudia
dc.contributor.authorMcCormick, Sheila
dc.date.accessioned2015-11-18T15:21:14Z
dc.date.available2015-11-18T15:21:14Z
dc.date.issued2015-10-27
dc.description.abstractAn Arabidopsis pollen grain (male gametophyte) consists of three cells: the vegetative cell, which forms the pollen tube, and two sperm cells enclosed within the vegetative cell. It is still unclear if there is intercellular communication between the vegetative cell and the sperm cells. Here we show that ABA-hypersensitive germination3 (AHG3), encoding a protein phosphatase, is specifically transcribed in the vegetative cell but predominantly translated in sperm cells. We used a series of deletion constructs and promoter exchanges to document transport of AHG3 transcripts from the vegetative cell to sperm and showed that their transport requires sequences in both the 5' UTR and the coding region. Thus, in addition its known role in transporting sperm during pollen tube growth, the vegetative cell also contributes transcripts to the sperm cells.pt_PT
dc.description.sponsorshipUS Department of Agriculture-Agricultural Research Service Current Research Information System Grant: (5335–21000–030–00D), European Research Council Starting Independent Researcher grant.pt_PT
dc.identifier.citationHua Jiang, Jun Yi, Leonor C. Boavida, Yuan Chen, Jörg D. Becker, Claudia Köhler, and Sheila McCormick Intercellular communication in Arabidopsis thaliana pollen discovered via AHG3 transcript movement from the vegetative cell to sperm PNAS 2015 112: 13378-13383.pt_PT
dc.identifier.doi10.1073/pnas.1510854112pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/500
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNational Academy of Sciencespt_PT
dc.relationThe other Side of the Mirror: mRNA Transport and Non-Cell-Autonomous Activity in the Male Germ Unit
dc.relation.publisherversionhttp://www.pnas.org/content/112/43/13378.longpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectpollenpt_PT
dc.subjectspermpt_PT
dc.subjectmRNA transportpt_PT
dc.subjectprotein phosphatase 2Cpt_PT
dc.subjectvegetative nucleuspt_PT
dc.titleIntercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to spermpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleThe other Side of the Mirror: mRNA Transport and Non-Cell-Autonomous Activity in the Male Germ Unit
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/EXPL%2FBIA-PLA%2F0244%2F2013/PT
oaire.citation.endPage13383pt_PT
oaire.citation.issue43pt_PT
oaire.citation.startPage13378pt_PT
oaire.citation.titleProceedings of the National Academy of Sciences of the United States of Americapt_PT
oaire.citation.volume112pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationa95750c0-6348-48fb-bec3-204365074c83
relation.isProjectOfPublication.latestForDiscoverya95750c0-6348-48fb-bec3-204365074c83

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