Revisiting the role of the mother centriole in centriole biogenesis

Rodrigues-Martins, A; Riparbelli, M; Callaini, G; Glover, D M; Bettencourt-Dias, M

http://hdl.handle.net/10400.7/955

Use this identifier to reference this record.

Name:	Description:	Size:	Format:
Rodrigues-Martins_2007.pdf		434.2 KB	Adobe PDF	Download

Send Feedback

Authors

Abstract(s)

Centrioles duplicate once in each cell division cycle through so-called templated or canonical duplication. SAK, also called PLK4 (SAK/PLK4), a kinase implicated in tumor development, is an upstream regulator of canonical biogenesis necessary for centriole formation. We found that overexpression of SAK/PLK4 could induce amplification of centrioles in Drosophila embryos and their de novo formation in unfertilized eggs. Both processes required the activity of DSAS-6 and DSAS-4, two molecules required for canonical duplication. Thus, centriole biogenesis is a template-free self-assembly process triggered and regulated by molecules that ordinarily associate with the existing centriole. The mother centriole is not a bona fide template but a platform for a set of regulatory molecules that catalyzes and regulates daughter centriole assembly.

Keywords

Animals Animals, Genetically Modified Centrioles Centrosome Drosophila Drosophila Proteins Embryo, Nonmammalian Embryonic Development Female Mitosis Oocytes