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Publication
Pre-mRNA splicing repression triggers abiotic stress signaling in plants
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| main article | 2.56 MB | Adobe PDF | ||
| supplementary materials 1 | 3.32 MB | Adobe PDF | ||
| supplementary materials 2 | 14.65 KB | Microsoft Excel XML | ||
| supplementary materials 3 | 98.01 KB | Adobe PDF | ||
| supplementary materials 4 | 187.51 KB | Adobe PDF |
Advisor(s)
Abstract(s)
Alternative splicing (AS) of precursor RNAs enhances transcriptome plasticity and proteome diversity in response to diverse growth and stress cues. Recent work has shown that AS is pervasive across plant species, with more than 60% of intron-containing genes producing different isoforms. Mammalian cell-based assays have discovered various inhibitors of AS. Here, we show that the macrolide pladienolide B (PB) inhibits constitutive splicing and AS in plants. Also, our RNA sequencing (RNA-seq) data revealed that PB mimics abiotic stress signals including salt, drought and abscisic acid (ABA). PB activates the abiotic stress- and ABA-responsive reporters RD29A::LUC and MAPKKK18::uidA in Arabidopsis thaliana and mimics the effects of ABA on stomatal aperture. Genome-wide analysis of AS by RNA-seq revealed that PB perturbs the splicing machinery and leads to a striking increase in intron retention and a reduction in other forms of AS. Interestingly, PB treatment activates the ABA signaling pathway by inhibiting the splicing of clade A PP2C phosphatases while still maintaining to some extent the splicing of ABA-activated SnRK2 kinases. Taken together, our data establish PB as an inhibitor and modulator of splicing and a mimic of abiotic stress signals in plants. Thus, PB reveals the molecular underpinnings of the interplay between stress responses, ABA signaling and post-transcriptional regulation in plants.
Description
This publication hasn't any creative commons license associated.
This deposit is composed by a publication in which the IGC's authors have had the role of collaboration (it's a collaboration publication). This type of deposit in ARCA is in restrictedAccess (it can't be in open access to the public), and can only be accessed by two ways: either by requesting a legal copy from the author (the email contact present in this deposit) or by visiting the following link: https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.13383
This work can also be accessed by visiting the following link: http://hdl.handle.net/10754/622750
This deposit is composed by a publication in which the IGC's authors have had the role of collaboration (it's a collaboration publication). This type of deposit in ARCA is in restrictedAccess (it can't be in open access to the public), and can only be accessed by two ways: either by requesting a legal copy from the author (the email contact present in this deposit) or by visiting the following link: https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.13383
This work can also be accessed by visiting the following link: http://hdl.handle.net/10754/622750
Keywords
alternative splicing pladienolide B splicing inhibitors serine/arginine-rich proteins abiotic stress responses abscisic acid
Citation
Ling, Y. , Alshareef, S. , Butt, H. , Lozano‐Juste, J. , Li, L. , Galal, A. A., Moustafa, A. , Momin, A. A., Tashkandi, M. , Richardson, D. N., Fujii, H. , Arold, S. , Rodriguez, P. L., Duque, P. and Mahfouz, M. M. (2017), Pre‐mRNA splicing repression triggers abiotic stress signaling in plants. Plant J, 89: 291-309. doi:10.1111/tpj.13383