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Pre-mRNA splicing repression triggers abiotic stress signaling in plants

2017-01Journal article Restricted access
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dc.contributor.authorLing, Yu
dc.contributor.authorAlshareef, Sahar
dc.contributor.authorButt, Haroon
dc.contributor.authorLozano-Juste, Jorge
dc.contributor.authorLi, Lixin
dc.contributor.authorGalal, Aya A.
dc.contributor.authorMoustafa, Ahmed
dc.contributor.authorMomin, Afaque A.
dc.contributor.authorTashkandi, Manal
dc.contributor.authorRichardson, Dale N.
dc.contributor.authorFujii, Hiroaki
dc.contributor.authorArold, Stefan
dc.contributor.authorRodriguez, Pedro L.
dc.contributor.authorDuque, Paula
dc.contributor.authorMahfouz, Magdy M.
dc.date.accessioned2018-04-06T15:49:47Z
dc.date.available2018-04-06T15:49:47Z
dc.date.issued2017-01
dc.descriptionThis publication hasn't any creative commons license associated.pt_PT
dc.descriptionThis deposit is composed by a publication in which the IGC's authors have had the role of collaboration (it's a collaboration publication). This type of deposit in ARCA is in restrictedAccess (it can't be in open access to the public), and can only be accessed by two ways: either by requesting a legal copy from the author (the email contact present in this deposit) or by visiting the following link: https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.13383pt_PT
dc.descriptionThis work can also be accessed by visiting the following link: http://hdl.handle.net/10754/622750pt_PT
dc.description.abstractAlternative splicing (AS) of precursor RNAs enhances transcriptome plasticity and proteome diversity in response to diverse growth and stress cues. Recent work has shown that AS is pervasive across plant species, with more than 60% of intron-containing genes producing different isoforms. Mammalian cell-based assays have discovered various inhibitors of AS. Here, we show that the macrolide pladienolide B (PB) inhibits constitutive splicing and AS in plants. Also, our RNA sequencing (RNA-seq) data revealed that PB mimics abiotic stress signals including salt, drought and abscisic acid (ABA). PB activates the abiotic stress- and ABA-responsive reporters RD29A::LUC and MAPKKK18::uidA in Arabidopsis thaliana and mimics the effects of ABA on stomatal aperture. Genome-wide analysis of AS by RNA-seq revealed that PB perturbs the splicing machinery and leads to a striking increase in intron retention and a reduction in other forms of AS. Interestingly, PB treatment activates the ABA signaling pathway by inhibiting the splicing of clade A PP2C phosphatases while still maintaining to some extent the splicing of ABA-activated SnRK2 kinases. Taken together, our data establish PB as an inhibitor and modulator of splicing and a mimic of abiotic stress signals in plants. Thus, PB reveals the molecular underpinnings of the interplay between stress responses, ABA signaling and post-transcriptional regulation in plants.pt_PT
dc.description.sponsorshipKing Abdullah University of Science and Technology; MINECO grant: (BIO2014-52537-R); FCT grant: (PTDC/BIA-PLA/1084/2014).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLing, Y. , Alshareef, S. , Butt, H. , Lozano‐Juste, J. , Li, L. , Galal, A. A., Moustafa, A. , Momin, A. A., Tashkandi, M. , Richardson, D. N., Fujii, H. , Arold, S. , Rodriguez, P. L., Duque, P. and Mahfouz, M. M. (2017), Pre‐mRNA splicing repression triggers abiotic stress signaling in plants. Plant J, 89: 291-309. doi:10.1111/tpj.13383pt_PT
dc.identifier.doi10.1111/tpj.13383pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/858
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationBIO2014-52537-Rpt_PT
dc.relationAlternative Splicing and SR Proteins in ABA-mediated Stress Responses
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.13383pt_PT
dc.subjectalternative splicingpt_PT
dc.subjectpladienolide Bpt_PT
dc.subjectsplicing inhibitorspt_PT
dc.subjectserine/arginine-rich proteinspt_PT
dc.subjectabiotic stress responsespt_PT
dc.subjectabscisic acidpt_PT
dc.titlePre-mRNA splicing repression triggers abiotic stress signaling in plantspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlternative Splicing and SR Proteins in ABA-mediated Stress Responses
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-PLA%2F1084%2F2014/PT
oaire.citation.endPage309pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage291pt_PT
oaire.citation.titlePlant Journalpt_PT
oaire.citation.volume89pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication863fd76f-7f4f-4663-81aa-a59b91dcad96
relation.isProjectOfPublication.latestForDiscovery863fd76f-7f4f-4663-81aa-a59b91dcad96

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