Methods to Study Centrosomes and Cilia in Drosophila

Jana, Swadhin Chandra; Mendonça, Susana; Werner, Sascha; Bettencourt-Dias, Monica

http://hdl.handle.net/10400.7/832

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Authors

Jana, Swadhin Chandra

Mendonça, Susana

Werner, Sascha

Bettencourt-Dias, Monica

Abstract(s)

Centrioles and cilia are highly conserved eukaryotic organelles. Drosophila melanogaster is a powerful genetic and cell biology model organism, extensively used to discover underlying mechanisms of centrosome and cilia biogenesis and function. Defects in centrosomes and cilia reduce fertility and affect different sensory functions, such as proprioception, olfaction, and hearing. The fly possesses a large diversity of ciliary structures and assembly modes, such as motile, immotile, and intraflagellar transport (IFT)-independent or IFT-dependent assembly. Moreover, all the diverse ciliated cells harbor centrioles at the base of the cilia, called basal bodies, making the fly an attractive model to better understand the biology of this organelle. This chapter describes protocols to visualize centrosomes and cilia by fluorescence and electron microscopy.

Description

The deposited item is a book chapter and is part of the series " Methods in Molecular Biology book series ([MIMB, volume 1454]) published by the publisher Humana Press.
The deposited book chapter is a pre-print version and hasn't been submitted to peer reviewing.
There is no public supplementary material available for this publication.
This publication hasn't any creative commons license associated.

Keywords

Drosophila Centrosome Basal body Cilia Intraflagellar transport Sensory neuron Sperm

URI

http://hdl.handle.net/10400.7/832

Citation

Jana S.C., Mendonça S., Werner S., Bettencourt-Dias M. (2016) Methods to Study Centrosomes and Cilia in Drosophila. In: Satir P., Christensen S. (eds) Cilia. Methods in Molecular Biology, vol 1454. Humana Press, New York, NY