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Methods to Study Centrosomes and Cilia in Drosophila

2016-08-12Preprint Open access
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dc.contributor.authorJana, Swadhin Chandra
dc.contributor.authorMendonça, Susana
dc.contributor.authorWerner, Sascha
dc.contributor.authorBettencourt-Dias, Monica
dc.date.accessioned2018-02-07T17:16:59Z
dc.date.available2018-02-07T17:16:59Z
dc.date.issued2016-08-12
dc.descriptionThe deposited item is a book chapter and is part of the series " Methods in Molecular Biology book series ([MIMB, volume 1454]) published by the publisher Humana Press.pt_PT
dc.descriptionThe deposited book chapter is a pre-print version and hasn't been submitted to peer reviewing.pt_PT
dc.descriptionThere is no public supplementary material available for this publication.pt_PT
dc.descriptionThis publication hasn't any creative commons license associated.pt_PT
dc.description.abstractCentrioles and cilia are highly conserved eukaryotic organelles. Drosophila melanogaster is a powerful genetic and cell biology model organism, extensively used to discover underlying mechanisms of centrosome and cilia biogenesis and function. Defects in centrosomes and cilia reduce fertility and affect different sensory functions, such as proprioception, olfaction, and hearing. The fly possesses a large diversity of ciliary structures and assembly modes, such as motile, immotile, and intraflagellar transport (IFT)-independent or IFT-dependent assembly. Moreover, all the diverse ciliated cells harbor centrioles at the base of the cilia, called basal bodies, making the fly an attractive model to better understand the biology of this organelle. This chapter describes protocols to visualize centrosomes and cilia by fluorescence and electron microscopy.pt_PT
dc.description.sponsorshipFundação Portuguesa para a Ciência e Tecnologia grants: (SFRH/BPD/87479/2012, SFRH/BD/52176/2013); EMBO installation grant; ERC starting grant.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJana S.C., Mendonça S., Werner S., Bettencourt-Dias M. (2016) Methods to Study Centrosomes and Cilia in Drosophila. In: Satir P., Christensen S. (eds) Cilia. Methods in Molecular Biology, vol 1454. Humana Press, New York, NYpt_PT
dc.identifier.doi10.1007/978-1-4939-3789-9_14pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/832
dc.language.isoengpt_PT
dc.peerreviewednopt_PT
dc.publisherHumana Presspt_PT
dc.relationDYNAMICS AND MAINTENANCE OF CILIA COMPONENTS
dc.relation.publisherversionhttps://link.springer.com/protocol/10.1007%2F978-1-4939-3789-9_14pt_PT
dc.subjectDrosophilapt_PT
dc.subjectCentrosomept_PT
dc.subjectBasal bodypt_PT
dc.subjectCiliapt_PT
dc.subjectIntraflagellar transportpt_PT
dc.subjectSensory neuronpt_PT
dc.subjectSpermpt_PT
dc.titleMethods to Study Centrosomes and Cilia in Drosophilapt_PT
dc.typepreprint
dspace.entity.typePublication
oaire.awardTitleDYNAMICS AND MAINTENANCE OF CILIA COMPONENTS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F87479%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F52176%2F2013/PT
oaire.citation.endPage236pt_PT
oaire.citation.startPage215pt_PT
oaire.citation.titleCiliapt_PT
oaire.citation.volume1454pt_PT
oaire.fundingStreamSFRH
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typepreprintpt_PT
relation.isProjectOfPublication12b42d08-d406-470c-a972-2ac4992a9b3e
relation.isProjectOfPublication43a5449e-6b12-42e3-8ca8-118bb81a32d5
relation.isProjectOfPublication.latestForDiscovery43a5449e-6b12-42e3-8ca8-118bb81a32d5

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