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A two-step mechanism for epigenetic specification of centromere identity and function

2013-07-21Journal article Open access
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dc.contributor.authorFachinetti, Daniele
dc.contributor.authorDiego Folco, H.
dc.contributor.authorNechemia-Arbely, Yael
dc.contributor.authorValente, Luis P.
dc.contributor.authorNguyen, Kristen
dc.contributor.authorWong, Alex J.
dc.contributor.authorZhu, Quan
dc.contributor.authorHolland, Andrew J.
dc.contributor.authorDesai, Arshad
dc.contributor.authorJansen, Lars E. T.
dc.contributor.authorCleveland, Don W.
dc.date.accessioned2016-06-07T15:08:11Z
dc.date.available2016-06-07T15:08:11Z
dc.date.issued2013-07-21
dc.description.abstractThe basic determinant of chromosome inheritance, the centromere, is specified in many eukaryotes by an epigenetic mark. Using gene targeting in human cells and fission yeast, chromatin containing the centromere-specific histone H3 variant CENP-A is demonstrated to be the epigenetic mark that acts through a two-step mechanism to identify, maintain and propagate centromere function indefinitely. Initially, centromere position is replicated and maintained by chromatin assembled with the centromere-targeting domain (CATD) of CENP-A substituted into H3. Subsequently, nucleation of kinetochore assembly onto CATD-containing chromatin is shown to require either the amino- or carboxy-terminal tail of CENP-A for recruitment of inner kinetochore proteins, including stabilizing CENP-B binding to human centromeres or direct recruitment of CENP-C, respectively.pt_PT
dc.description.sponsorshipNational Institutes of Health grant: (GM 074150); Ludwig Institute for Cancer Research; European Molecular Biology Organization (EMBO) long-term fellowship.pt_PT
dc.identifier.citationFachinetti, D., Diego Folco, H., Nechemia-Arbely, Y., Valente, L. P., Nguyen, K., Wong, A. J., Zhu, Q., Holland, A. J., Desai, A., Jansen, L. E. T., Cleveland, D. W. (2013). A two-step mechanism for epigenetic specification of centromere identity and function. Nat Cell Biol, 15(9), 1056–1066.pt_PT
dc.identifier.doi10.1038/ncb2805pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/625
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNational Academy of Sciencespt_PT
dc.relation.publisherversionhttp://www.nature.com/ncb/journal/v15/n9/full/ncb2805.htmlpt_PT
dc.subjectAdenoviridaept_PT
dc.subjectAutoantigenspt_PT
dc.subjectCentromerept_PT
dc.subjectCentromere Protein Bpt_PT
dc.subjectChromatinpt_PT
dc.subjectChromosomal Proteins, Non-Histonept_PT
dc.subjectEpithelial Cellspt_PT
dc.subjectGenetic Vectorspt_PT
dc.subjectHistonespt_PT
dc.subjectHumanspt_PT
dc.subjectProtein Structure, Tertiarypt_PT
dc.subjectRetinapt_PT
dc.subjectSchizosaccharomycespt_PT
dc.subjectSchizosaccharomyces pombe Proteinspt_PT
dc.subjectSignal Transductionpt_PT
dc.subjectEpigenesis, Geneticpt_PT
dc.titleA two-step mechanism for epigenetic specification of centromere identity and functionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1066pt_PT
oaire.citation.issue9pt_PT
oaire.citation.startPage1056pt_PT
oaire.citation.titleNature Cell Biologypt_PT
oaire.citation.volume15pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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