Highly dynamic host actin reorganization around developing Plasmodium inside hepatocytes

Gomes-Santos, Carina S S; Itoe, Maurice A; Afonso, Cristina; Henriques, Ricardo; Gardner, Rui; Sepúlveda, Nuno; Simões, Pedro D; Raquel, Helena; Almeida, António Paulo; Moita, Luis F; Frischknecht, Friedrich; Mota, Maria M

http://hdl.handle.net/10400.7/649

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Autores

Gomes-Santos, Carina S S

Almeida, António Paulo

Moita, Luis F

Resumo(s)

Plasmodium sporozoites are transmitted by Anopheles mosquitoes and infect hepatocytes, where a single sporozoite replicates into thousands of merozoites inside a parasitophorous vacuole. The nature of the Plasmodium-host cell interface, as well as the interactions occurring between these two organisms, remains largely unknown. Here we show that highly dynamic hepatocyte actin reorganization events occur around developing Plasmodium berghei parasites inside human hepatoma cells. Actin reorganization is most prominent between 10 to 16 hours post infection and depends on the actin severing and capping protein, gelsolin. Live cell imaging studies also suggest that the hepatocyte cytoskeleton may contribute to parasite elimination during Plasmodium development in the liver.

Palavras-chave

Actin Cytoskeleton Actins Animals Cells, Cultured Gelsolin Green Fluorescent Proteins Hepatocytes Host-Parasite Interactions Humans Kinetics Liver Mice Mice, Inbred BALB C Organisms, Genetically Modified Plasmodium Protein Multimerization Tubulin

URI

http://hdl.handle.net/10400.7/649

Citação

Gomes-Santos CSS, Itoe MA, Afonso C, Henriques R, Gardner R, et al. (2012) Highly Dynamic Host Actin Reorganization around Developing Plasmodium Inside Hepatocytes. PLoS ONE 7(1): e29408. doi:10.1371/journal.pone.0029408