Highly dynamic host actin reorganization around developing Plasmodium inside hepatocytes

Gomes-Santos, Carina S S; Itoe, Maurice A; Afonso, Cristina; Henriques, Ricardo; Gardner, Rui; Sepúlveda, Nuno; Simões, Pedro D; Raquel, Helena; Almeida, António Paulo; Moita, Luis F; Frischknecht, Friedrich; Mota, Maria M

http://hdl.handle.net/10400.7/649

Use this identifier to reference this record.

Name:	Description:	Size:	Format:
journal.pone.0029408.PDF	artigo principal	1.94 MB	Adobe PDF	Download
Figure_S1.tif	material suplementar 1	1.88 MB	TIFF	Download
Figure_S2.tif	material suplementar 2	606.63 KB	TIFF	Download
Figure_S3.tif	material suplementar 3	1.85 MB	TIFF	Download
Movie_S1.mov	material suplementar 4	309.14 KB	Video Quicktime	Download

Send Feedback

Authors

Gomes-Santos, Carina S S

Almeida, António Paulo

Moita, Luis F

Abstract(s)

Plasmodium sporozoites are transmitted by Anopheles mosquitoes and infect hepatocytes, where a single sporozoite replicates into thousands of merozoites inside a parasitophorous vacuole. The nature of the Plasmodium-host cell interface, as well as the interactions occurring between these two organisms, remains largely unknown. Here we show that highly dynamic hepatocyte actin reorganization events occur around developing Plasmodium berghei parasites inside human hepatoma cells. Actin reorganization is most prominent between 10 to 16 hours post infection and depends on the actin severing and capping protein, gelsolin. Live cell imaging studies also suggest that the hepatocyte cytoskeleton may contribute to parasite elimination during Plasmodium development in the liver.

Keywords

Actin Cytoskeleton Actins Animals Cells, Cultured Gelsolin Green Fluorescent Proteins Hepatocytes Host-Parasite Interactions Humans Kinetics Liver Mice Mice, Inbred BALB C Organisms, Genetically Modified Plasmodium Protein Multimerization Tubulin

URI

http://hdl.handle.net/10400.7/649

Citation

Gomes-Santos CSS, Itoe MA, Afonso C, Henriques R, Gardner R, et al. (2012) Highly Dynamic Host Actin Reorganization around Developing Plasmodium Inside Hepatocytes. PLoS ONE 7(1): e29408. doi:10.1371/journal.pone.0029408