dc.contributor.author	Carvalho-Santos, Z.
dc.contributor.author	Machado, P.
dc.contributor.author	Branco, P.
dc.contributor.author	Tavares-Cadete, F.
dc.contributor.author	Rodrigues-Martins, A.
dc.contributor.author	Pereira-Leal, J. B.
dc.contributor.author	Bettencourt-Dias, M.
dc.date.accessioned	2016-12-02T13:58:07Z
dc.date.available	2016-12-02T13:58:07Z
dc.date.issued	2010-04-14
dc.description.abstract	The centriole and basal body (CBB) structure nucleates cilia and flagella, and is an essential component of the centrosome, underlying eukaryotic microtubule-based motility, cell division and polarity. In recent years, components of the CBB-assembly machinery have been identified, but little is known about their regulation and evolution. Given the diversity of cellular contexts encountered in eukaryotes, but the remarkable conservation of CBB morphology, we asked whether general mechanistic principles could explain CBB assembly. We analysed the distribution of each component of the human CBB-assembly machinery across eukaryotes as a strategy to generate testable hypotheses. We found an evolutionarily cohesive and ancestral module, which we term UNIMOD and is defined by three components (SAS6, SAS4/CPAP and BLD10/CEP135), that correlates with the occurrence of CBBs. Unexpectedly, other players (SAK/PLK4, SPD2/CEP192 and CP110) emerged in a taxon-specific manner. We report that gene duplication plays an important role in the evolution of CBB components and show that, in the case of BLD10/CEP135, this is a source of tissue specificity in CBB and flagella biogenesis. Moreover, we observe extreme protein divergence amongst CBB components and show experimentally that there is loss of cross-species complementation among SAK/PLK4 family members, suggesting species-specific adaptations in CBB assembly. We propose that the UNIMOD theory explains the conservation of CBB architecture and that taxon- and tissue-specific molecular innovations, gained through emergence, duplication and divergence, play important roles in coordinating CBB biogenesis and function in different cellular contexts.	pt_PT
dc.description.sponsorship	Fundação Calouste Gulbenkian; Fundação para a Ciência e Tecnologia scholarships and grants: (POCI2010); Câmara Municipal de Oeiras; EMBO Installation Grant.	pt_PT
dc.identifier.citation	Stepwise evolution of the centriole-assembly pathway Zita Carvalho-Santos, Pedro Machado, Pedro Branco, Filipe Tavares-Cadete, Ana Rodrigues-Martins, José B. Pereira-Leal, Mónica Bettencourt-Dias J Cell Sci 2010 123: 1414-1426; doi: 10.1242/jcs.064931	pt_PT
dc.identifier.doi	10.1242/jcs.064931	pt_PT
dc.identifier.uri	http://hdl.handle.net/10400.7/726
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	Company of Biologists	pt_PT
dc.relation	Regulation Of The Function Of The Tumourigenesis-Associated Kinase SAK/PLK4
dc.relation.publisherversion	http://jcs.biologists.org/content/123/9/1414	pt_PT
dc.subject	Centriole	pt_PT
dc.subject	Basal body	pt_PT
dc.subject	Evolution	pt_PT
dc.subject	Drosophila	pt_PT
dc.subject	Flagella	pt_PT
dc.subject	Comparative genomics	pt_PT
dc.title	Stepwise evolution of the centriole-assembly pathway	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.awardTitle	Regulation Of The Function Of The Tumourigenesis-Associated Kinase SAK/PLK4
oaire.awardURI	info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-BCM%2F73195%2F2006/PT
oaire.awardURI	info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-OBD%2F73194%2F2006/PT
oaire.citation.endPage	1426	pt_PT
oaire.citation.issue	9	pt_PT
oaire.citation.startPage	1414	pt_PT
oaire.citation.title	Journal of Cell Science	pt_PT
oaire.citation.volume	123	pt_PT
oaire.fundingStream	3599-PPCDT
oaire.fundingStream	3599-PPCDT
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.name	Fundação para a Ciência e a Tecnologia
project.funder.name	Fundação para a Ciência e a Tecnologia
rcaap.rights	openAccess	pt_PT
rcaap.type	article	pt_PT
relation.isProjectOfPublication	9b2b6cfa-a671-4198-93bd-04b7b4e383c0
relation.isProjectOfPublication	665506fd-76ee-424f-adb1-84f7fa42033b
relation.isProjectOfPublication.latestForDiscovery	9b2b6cfa-a671-4198-93bd-04b7b4e383c0

Stepwise evolution of the centriole-assembly pathway

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