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Stepwise evolution of the centriole-assembly pathway

dc.contributor.authorCarvalho-Santos, Z.
dc.contributor.authorMachado, P.
dc.contributor.authorBranco, P.
dc.contributor.authorTavares-Cadete, F.
dc.contributor.authorRodrigues-Martins, A.
dc.contributor.authorPereira-Leal, J. B.
dc.contributor.authorBettencourt-Dias, M.
dc.date.accessioned2016-12-02T13:58:07Z
dc.date.available2016-12-02T13:58:07Z
dc.date.issued2010-04-14
dc.description.abstractThe centriole and basal body (CBB) structure nucleates cilia and flagella, and is an essential component of the centrosome, underlying eukaryotic microtubule-based motility, cell division and polarity. In recent years, components of the CBB-assembly machinery have been identified, but little is known about their regulation and evolution. Given the diversity of cellular contexts encountered in eukaryotes, but the remarkable conservation of CBB morphology, we asked whether general mechanistic principles could explain CBB assembly. We analysed the distribution of each component of the human CBB-assembly machinery across eukaryotes as a strategy to generate testable hypotheses. We found an evolutionarily cohesive and ancestral module, which we term UNIMOD and is defined by three components (SAS6, SAS4/CPAP and BLD10/CEP135), that correlates with the occurrence of CBBs. Unexpectedly, other players (SAK/PLK4, SPD2/CEP192 and CP110) emerged in a taxon-specific manner. We report that gene duplication plays an important role in the evolution of CBB components and show that, in the case of BLD10/CEP135, this is a source of tissue specificity in CBB and flagella biogenesis. Moreover, we observe extreme protein divergence amongst CBB components and show experimentally that there is loss of cross-species complementation among SAK/PLK4 family members, suggesting species-specific adaptations in CBB assembly. We propose that the UNIMOD theory explains the conservation of CBB architecture and that taxon- and tissue-specific molecular innovations, gained through emergence, duplication and divergence, play important roles in coordinating CBB biogenesis and function in different cellular contexts.pt_PT
dc.description.sponsorshipFundação Calouste Gulbenkian; Fundação para a Ciência e Tecnologia scholarships and grants: (POCI2010); Câmara Municipal de Oeiras; EMBO Installation Grant.pt_PT
dc.identifier.citationStepwise evolution of the centriole-assembly pathway Zita Carvalho-Santos, Pedro Machado, Pedro Branco, Filipe Tavares-Cadete, Ana Rodrigues-Martins, José B. Pereira-Leal, Mónica Bettencourt-Dias J Cell Sci 2010 123: 1414-1426; doi: 10.1242/jcs.064931pt_PT
dc.identifier.doi10.1242/jcs.064931pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/726
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherCompany of Biologistspt_PT
dc.relationRegulation Of The Function Of The Tumourigenesis-Associated Kinase SAK/PLK4
dc.relation.publisherversionhttp://jcs.biologists.org/content/123/9/1414pt_PT
dc.subjectCentriolept_PT
dc.subjectBasal bodypt_PT
dc.subjectEvolutionpt_PT
dc.subjectDrosophilapt_PT
dc.subjectFlagellapt_PT
dc.subjectComparative genomicspt_PT
dc.titleStepwise evolution of the centriole-assembly pathwaypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleRegulation Of The Function Of The Tumourigenesis-Associated Kinase SAK/PLK4
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-BCM%2F73195%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-OBD%2F73194%2F2006/PT
oaire.citation.endPage1426pt_PT
oaire.citation.issue9pt_PT
oaire.citation.startPage1414pt_PT
oaire.citation.titleJournal of Cell Sciencept_PT
oaire.citation.volume123pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication9b2b6cfa-a671-4198-93bd-04b7b4e383c0
relation.isProjectOfPublication665506fd-76ee-424f-adb1-84f7fa42033b
relation.isProjectOfPublication.latestForDiscovery9b2b6cfa-a671-4198-93bd-04b7b4e383c0

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